Sample preparation and orthogonal chromatography for broad polarity range plasma metabolomics: Application to human subjects with neurodegenerative dementia

• Published in: Analytical biochemistry Vol. 455; pp. 48 - 54
• Main Authors: Armirotti, Andrea, Basit, Abdul, Realini, Natalia, Caltagirone, Carlo, Bossù, Paola, Spalletta, Gianfranco, Piomelli, Daniele
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.06.2014
• Subjects: Aged; Alzheimer disease; Alzheimer Disease - blood; Animals; Biochemistry - methods; Blood Proteins - analysis; Case-Control Studies; chromatography; Chromatography, Liquid - methods; cognitive disorders; Dementia - blood; Dried plasma spot; Female; Humans; hydrophilicity; hydrophobicity; Male; Mass spectrometry; metabolome; Metabolomics; Metabolomics - methods; Mice; Multivariate Analysis; Orthogonal chromatography; Sample preparation; Sphingomyelins - blood; Tandem Mass Spectrometry; Metabolomics; Dried plasma spot; Sample preparation; Mass spectrometry; Orthogonal chromatography
• ISSN: 0003-2697; 1096-0309; 1096-0309
• DOI: 10.1016/j.ab.2014.03.019

We describe a simple protocol for the preparation and orthogonal hydrophobic/hydrophilic LC–MS/MS analysis of mouse and human plasma samples, which enables the untargeted (“shotgun”) or targeted profiling of hydrophilic, amphipathic, and hydrophobic constituents of plasma metabolome. The protocol is rapid, efficient, and reliable, and offers several advantages compared to current procedures. When applied to a training set of human plasma samples, the protocol allowed for the rapid acquisition of full LogP metabolic profiles in plasma samples obtained from cognitively healthy human subjects and age-matched subjects with mild cognitive impairment or Alzheimer’s disease (n=15 each). Targeted analyses confirmed these findings, which are consistent with data previously published by other groups.