The development of a MIP-optosensor for the detection of monoamine naphthalenes in drinking water

• Published in: Biosensors & bioelectronics Vol. 24; no. 7; pp. 2305 - 2311
• Main Authors: Valero-Navarro, Angel, Salinas-Castillo, Alfonso, Fernández-Sánchez, Jorge F., Segura-Carretero, Antonio, Mallavia, Ricardo, Fernández-Gutiérrez, Alberto
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 15.03.2009; Elsevier
• Subjects: Biological and medical sciences; Biosensing Techniques - instrumentation; Biotechnology; Computer-Aided Design; Environmental Monitoring - instrumentation; Equipment Design; Equipment Failure Analysis; Flow injection; Flow Injection Analysis - instrumentation; Fluorescence optosensor; Fundamental and applied biological sciences. Psychology; Molecular imprinting; Naphthalene compounds; Naphthalenes - analysis; Optical Devices; Reproducibility of Results; Sensitivity and Specificity; Water analysis; Water Pollutants, Chemical - analysis; Water Supply - analysis; Water analysis; Molecular imprinting; Fluorescence optosensor; Naphthalene compounds; Flow injection; Drinking water; Naphthalene; Fluorescence; Monoamine; Aromatic compound; Detection
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2008.11.022

To enhance the advantages of fluorescent flow-through sensing for drinking water we have designed a novel sensing matrix based on molecularly imprinted polymers (MIPs). The synergic combination of a tailor-made MIP recognition with a selective room temperature fluorescence detection is a novel concept for optosensing devices and is assessed here for the simple and selective determination of pollutants in water. We describe a simple approach to preparing synthetic receptors for monoamine naphthalene compounds (MA-NCs) using non-covalent molecular imprinting techniques and naphthalene as template. We examine in detail the binding characteristics of the imprinted polymer and describe the flow-through sensor of MA-NCs by solid-surface fluorescence. Its detection limits for recognizing 1-naphthylamine (1-NA) and 2-naphthylamine (2-NA) separately are 26ngmL−1 and 50ngmL−1, respectively, and it also determines 1-NA and 2-NA simultaneously with a detection limit of 45ngmL−1. All the instrumental, chemical and flow variables were carefully optimized and an interference study was carried out to demonstrate its applicability and selectivity. Finally, we applied it to the analysis of 1-NA and 2-NA in tap and mineral waters, obtaining a 98% average recovery rate.