Long-lived IgE- and IgG-secreting cells in rodents manifesting persistent antibody responses

BALB/c mice and BN rats manifesting persistent IgE and IgG responses were examined up to 1 year after immunization. A significant proportion of the ongoing antibody response in these animals survived lethal X-irradiation employing dosages sufficient to deplete B memory cells. The persistent IgE resp...

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Bibliographic Details
Published inCellular immunology Vol. 89; no. 2; pp. 281 - 289
Main Authors Holt, P.G., Sedgwick, J.D., O'Leary, C., Krska, K., Leivers, S.
Format Journal Article
LanguageEnglish
Published San Diego, CA Elsevier Inc 01.12.1984
Elsevier
Subjects
Analysis of the immune response. Humoral and cellular immunity
Animals
Antibody Formation - radiation effects
Antibody-Producing Cells - immunology
Antibody-Producing Cells - radiation effects
Biological and medical sciences
Enzyme-Linked Immunosorbent Assay
Female
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Immunobiology
Immunoglobulin E - analysis
Immunoglobulin G - analysis
Male
Mice
Mice, Inbred BALB C
Organs and cells involved in the immune response
Rats
Rats, Inbred Strains
Species Specificity
Time Factors
Persistence
Vertebrata
Immunization
Mammalia
Immune response
Rat
Antibody
IgE
Rodentia
IgG
ELISA assay
Humoral immunity
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Summary:BALB/c mice and BN rats manifesting persistent IgE and IgG responses were examined up to 1 year after immunization. A significant proportion of the ongoing antibody response in these animals survived lethal X-irradiation employing dosages sufficient to deplete B memory cells. The persistent IgE responses in both species were refractory to exogenous isotype-specific suppressor cells taken from tolerant syngeneic animals, which were shown to abrogate primary IgE responses in parallel tests. Employing a novel ELISA-based assay for plaque forming cells, long-lived radioresistant IgE- and IgG-secreting cells were identified in differing ratios in lymph nodes, spleen, and bone marrow of both species. These long-lived cells were shown to arise following maximum antigenic challenge with antigen plus adjuvant, and after repeated low-grade stimulation by antigen alone, including passive inhalation of dilute antigen aerosols.
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ISSN:0008-8749
1090-2163
DOI:10.1016/0008-8749(84)90330-7