Effect of ischemia preconditioning on renal ischemia/reperfusion injury in rats

To study the effect of ischemia preconditioning (IP) on renal ischemia/reperfusion (I/R)-associated functional injury and expression of renal adhesion molecules in rats. The ischemia preconditioning plan adopted in this experiment involved renal warm ischemia for 6 min. and blood flow for 4 min., re...

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Published inInternational Brazilian Journal of Urology Vol. 38; no. 6; pp. 842 - 854
Main Authors Fan, Lian-hui, He, Long, Cao, Zhi-qiang, Xiang, Bjun, Liu, Long
Format Journal Article
LanguageEnglish
Published Brazil Sociedade Brasileira de Urologia 01.12.2012
Subjects
Animals
Cell Adhesion Molecules - analysis
Creatinine - blood
Disease Models, Animal
E-Selectin
E-Selectin - analysis
Female
Immunohistochemistry
Intercellular Adhesion Molecule-1
Ischemia
Ischemic Preconditioning
Ischemic Preconditioning - methods
Kidney - blood supply
Kidney - pathology
Male
P-Selectin
P-Selectin - analysis
Rats
Rats, Wistar
Reperfusion Injury
Reperfusion Injury - pathology
Reperfusion Injury - prevention & control
Time Factors
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Summary:To study the effect of ischemia preconditioning (IP) on renal ischemia/reperfusion (I/R)-associated functional injury and expression of renal adhesion molecules in rats. The ischemia preconditioning plan adopted in this experiment involved renal warm ischemia for 6 min. and blood flow for 4 min., repeated four times. The Wistar rat kidneys used for warm ischemia preconditioning were subjected to 60 min of renal warm ischemia followed by reperfusion. The rat kidneys with ischemia/reperfusion were compared with the ischemia preconditioning group to observe rat renal function and changes in the expression of renal adhesion molecules ICAM-1, P-Selectin, and E-Selectin. The expression of rat renal adhesion molecules (ICAM-1, P-Selectin, and E-Selectin) with ischemia preconditioning was significantly lower than that of the ischemia/reperfusion group. Serum creatinine was significantly lower than that in the ischemia/reperfusion group after 48 hours. Ischemia preconditioning has a protective effect on renal function. Reduced expression of renal adhesion molecules is likely a mechanism involved in the observed protection.
ISSN:1677-5538
1677-6119
1677-6119
1677-5538
DOI:10.1590/1677-553820133806842