Flavobacterium indicum sp. nov., isolated from warm spring water in Assam, India

• Published in: International journal of systematic and evolutionary microbiology Vol. 56; no. 11; pp. 2617 - 2621
• Main Authors: Saha, P, Chakrabarti, T
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.11.2006; Society for General Microbiology
• Subjects: Animal, plant and microbial ecology; Anti-Bacterial Agents - pharmacology; Bacterial Typing Techniques; Bacteriology; Base Composition; Biological and medical sciences; Carbohydrate Metabolism; Culture Media; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Fatty Acids - analysis; Fatty Acids - chemistry; Flavobacterium; Flavobacterium - classification; Flavobacterium - cytology; Flavobacterium - isolation & purification; Flavobacterium - physiology; Flavobacterium aquatile; Flavobacterium columnare; Flavobacterium indicum; Flexibacter; Freshwater; Fundamental and applied biological sciences. Psychology; Genes, rRNA; Hot Springs - microbiology; India; Microbial ecology; Microbial Sensitivity Tests; Microbiology; Molecular Sequence Data; Phylogeny; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Sequence Homology, Nucleic Acid; Systematics; Various environments (extraatmospheric space, air, water); Water Microbiology; Asia; India; India, Assam; Flavobacterium; Flavobacteriaceae; Taxonomy; Bacteria; New species; Hot source; Assam India; Water spring
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.64309-0

Microbial Type Culture Collection and Gene Bank (MTCC), Institute of Microbial Technology (IMTECH), Sector 39A, Chandigarh 160 036, India Correspondence T. Chakrabarti tapan{at}imtech.res.in A polyphasic taxonomic approach was employed to characterize a strain designated GPTSA100-9 T , which was isolated from water sampled from a warm spring. The micro-organism, comprising Gram-negative, strictly aerobic rods, could not grow on nutritionally rich media such as tryptic soy broth. Analysis of the 16S rRNA gene sequence (1396 nt) of strain GPTSA100-9 T revealed that it is a member of the genus Flavobacterium , sharing 99.8 % sequence similarity with the CFB group bacterium strain A0653 (AF236016 ), 93.4 % with ‘[ Flexibacter ] aurantiacus subsp. excathedrus ’ and 93.2–92.0 % with Flavobacterium saliperosum , Flavobacterium soli , Flavobacterium aquatile and Flavobacterium columnare . The G+C content of the genomic DNA was 31.0 mol%. The major fatty acids of the strain grown on modified R2A agar were iso-C 15 : 0 (18.5 %), iso-C 15 : 1 G (18.0 %), summed feature 3 (iso-C 15 : 0 2-OH and/or C 16 : 1 7 c , 16.6 %) and iso-C 17 : 0 3-OH (9.0 %). On the basis of phenotypic and genotypic characteristics, strain GPTSA100-9 T represents a novel species of the genus Flavobacterium , for which the name Flavobacterium indicum sp. nov. is proposed. The type strain is GPTSA100-9 T (=MTCC 6936 T =DSM 17447 T ). The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain GPTSA100-9 T is AY904351 .