Micro-CT and Histomorphometric Study of Bone Regeneration Effect with Autogenous Tooth Biomaterial Enriched with Platelet-Rich Fibrin in an Animal Model

• Published in: Scanning Vol. 2021; pp. 1 - 7
• Main Authors: Lee, Yoon-Ki, Wadhwa, Puneet, Cai, HongXin, Jung, Sung-Uk, Zhao, Bing Cheng, Rim, Jae-Suk, Kim, Dong-Hyuck, Jang, Hyon-Seok, Lee, Eui-Seok
• Format: Journal Article
• Language: English
• Published: Mahwah Hindawi 2021; Hindawi Limited
• Subjects: Biocompatibility; Biomedical materials; Blood platelets; Bones; Collagen; Committees; Computed tomography; Defects; Enamel; Fibrin; Grafting; Growth factors; Hydroxyapatite; Rabbits; Regeneration (physiology); Skin & tissue grafts; Substitute bone; Surgery; Teeth; New Zealand; Belgium
• ISSN: 0161-0457; 1932-8745
• DOI: 10.1155/2021/6656791

The aim of this study was to evaluate the potential of tooth biomaterials as bone graft biomaterials for bone healing in rabbits. We prepared tooth biomaterial and platelet-rich fibrin (PRF) to fill the round-shaped defect in the skull of New Zealand white rabbits. These cranial defects were treated with different conditions as follows: group 1, a mixture of tooth biomaterials and platelet-rich fibrin (PRF); group 2, only tooth biomaterials; group 3, only PRF; and group 4, the unfilled control group. Specimens of the filled sites were harvested for analysis with microscopic computerized tomography (micro-CT) and histomorphology at 4 and 8 weeks. As a result of micro-CT, at 4 weeks, the bone volume percentages in groups 1 and 2 were 50.33±6.35 and 57.74±3.13, respectively, and that in the unfilled control group was 42.20±10.53 (p=0.001). At 8 weeks, the bone volume percentages in groups 1 and 2 were 53.73±9.60 and 54.56±8.44, respectively, and that in the unfilled control group was 37.86±7.66 (p=0.002). The difference between the experimental group 3 and the unfilled control group was not statistically significant. Histomorphologically, the total new bone was statistically different.