Isolation and characterization of microsatellite markers from the Mediterranean fruit fly, Ceratitis capitata: cross-species amplification in other Tephritidae species reveals a varying degree of transferability

• Published in: Molecular genetics and genomics : MGG Vol. 282; no. 3; pp. 283 - 306
• Main Authors: Stratikopoulos, E. E, Augustinos, A. A, Pavlopoulos, I. D, Economou, K. Ph, Mintzas, A, Mathiopoulos, K. D, Zacharopoulou, Antigone
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.09.2009; Springer-Verlag; Springer Nature B.V
• Subjects: Animal Genetics and Genomics; Animals; Base Sequence; Biochemistry; Biomedical and Life Sciences; Ceratitis capitata; Ceratitis capitata - classification; Ceratitis capitata - genetics; Chromosome Mapping; Chromosomes, Plant - genetics; Cloning; Fruits; Genetics; Genomes; Genomics; Human Genetics; Hybridization; In Situ Hybridization, Fluorescence; Laboratories; Life Sciences; Microbial Genetics and Genomics; Microsatellite Repeats - genetics; Molecular Sequence Data; Original Paper; Plant Genetics and Genomics; Polymorphism, Genetic; Sequence Analysis, DNA; Tephritidae; Tephritidae - classification; Tephritidae - genetics; Greece; Cross-species amplification; Microsatellites; Medfly; In situ hybridization; Tephritidae
• ISSN: 1617-4615; 1617-4623
• DOI: 10.1007/s00438-009-0465-3

The Mediterranean fruit fly, Ceratitis capitata, is a pest of major economic importance and has become a model for the development of SIT control programs for insect pests. Significant information has been accumulated on classical and population genetics of this species during the past 2 decades. However, the availability of molecular markers is limited. Here, we present the isolation and characterization of 159 microsatellite clones and the development of 108 polymorphic microsatellite markers for this insect pest. Mapping by in situ hybridization to polytene chromosomes of 21 microsatellite clones enriched the cytogenetic map that was previously constructed by our group. The enriched map provides a large number of STSs for future genome mapping projects. Cross-species amplification of these microsatellite loci in 12 Tephritidae species and sequence analysis of several amplification products indicated a varying degree of transferability and their possible usefulness as molecular and genetic markers in these species where genetic and molecular tools are limited.