Lymphocytes modulate peritoneal leukocyte recruitment in peritonitis

• Published in: Inflammation research Vol. 58; no. 9; pp. 553 - 560
• Main Authors: Kipari, T, Watson, S, Houlberg, K, Lepage, S, Hughes, J, Cailhier, Jean-François
• Format: Journal Article
• Language: English
• Published: Basel Basel : SP Birkhäuser Verlag Basel 01.09.2009; SP Birkhäuser Verlag Basel; Springer Nature B.V
• Subjects: Allergology; Animals; B-Lymphocytes - immunology; Biomedical and Life Sciences; Biomedicine; Chemokine CCL2 - genetics; Chemokine CCL2 - immunology; Chemokines - immunology; Dermatology; Homeodomain Proteins - genetics; Homeodomain Proteins - metabolism; Immunology; Interleukin-6 - genetics; Interleukin-6 - immunology; Leukocytes - cytology; Leukocytes - immunology; Lymphocytes - cytology; Lymphocytes - immunology; Mice; Mice, Inbred C57BL; Mice, Knockout; Mice, Nude; Neurology; Original Research Paper; Peritoneum - cytology; Peritoneum - immunology; Peritonitis - immunology; Pharmacology/Toxicology; Rheumatology; T-Lymphocytes - immunology; Lymphocyte deficient mice; Leukocyte recruitment; Lymphocytes; Experimental peritonitis; Chemokines
• ISSN: 1023-3830; 1420-908X; 1420-908X
• DOI: 10.1007/s00011-009-0019-5

Objective and design To investigate the modulating role of lymphocytes in leukocyte recruitment in a murine model of peritonitis. Materials or subjects RAG-1 knockout (KO) mice, NUDE mice and μMT KO mice were compared to their wild-type controls. Treatment Mice were administered with 1 ml of Brewer's thioglycollate (BTG) and terminal peritoneal lavages were performed at 8, 24, 72 and 120 h after treatment. Methods Leukocyte numbers recruited at the different time points following a BTG administration were determined. Chemokine and cytokine levels were assessed by either ELISAs or cytometric bead array. Results RAG-1 KO mice (absent B and T cells) exhibited increased early neutrophil infiltration and blunted late monocyte/macrophage infiltration. NUDE mice (absent T cells) exhibited both increased neutrophil and monocyte/macrophage infiltration. In contrast, μMT KO mice (absent B cells) demonstrated reduced influx of both neutrophils and monocyte/macrophages. Chemokine analysis revealed various differences in important chemokines. Conclusions These data suggest that T cells act to suppress leukocyte recruitment while B cells promote leukocyte recruitment.