Evaluation of selective plating media for the detection of heat- or freeze-injured Staphylococcus aureus

• Published in: Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) Vol. 43; no. 4; pp. 239 - 242
• Main Authors: "Onoue, Y. (Kanagawa-ken. Environmental Center, Yokohama (Japan)), Shindo, R, Teranishi, H, Furukawa, I, Hasegawa, Y, Maruyama, T.
• Format: Journal Article
• Language: Japanese
• Published: Japan Japanese Society for Food Hygiene and Safety 01.08.2002
• Subjects: Bacteriological Techniques; Baird-Parker agar; CELL COUNTING; CULTURE MEDIA; Freezing; heat-injury; Hot Temperature; mannitol-salt-egg yolk agar; STAPHYLOCOCCUS AUREUS; Staphylococcus aureus - isolation & purification; Staphylococcus aureus - physiology
• ISSN: 0015-6426; 1882-1006
• DOI: 10.3358/shokueishi.43.239

"The efficacy of Baird-Parker (BP) agar, mannitol-salt-egg yolk (MSEY) agar and mannitol salt (MS) agar in detecting Staphylococcus aureus FRI-100 heated at 52degC for 20 min in 100mmol/L potassium phosphate buffer was determined. Brain heart infusion agar with 1% pyruvate (BHIP agar) supported the highest recovery of injured cells and was used as the control medium. Of the three selective media, significantly higher recovery of heat-injured cells was observed on BP agar than MSEY agar, and the poorest recovery was observed on MS agar (p<0.05). Low recovery of unheated cells was obtained for MS compared with other media (p<0.05). A reduction in populations occurred gradually in reagent-grade water stored for 14 days at - 20degC There was no significant difference between BHIP agar and MS agar in the number of freeze-injured cells recovered from 1 to 14 days."