Halalkalicoccus jeotgali sp. nov., a halophilic archaeon from shrimp jeotgal, a traditional Korean fermented seafood

• Published in: International journal of systematic and evolutionary microbiology Vol. 57; no. 10; pp. 2296 - 2298
• Main Authors: Roh, Seong Woon, Nam, Young-Do, Chang, Ho-Won, Sung, Youlboong, Kim, Kyoung-Ho, Oh, Hee-Mock, Bae, Jin-Woo
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.10.2007; Society for General Microbiology
• Subjects: Animals; Bacteriology; Base Composition; Biological and medical sciences; Decapoda; DNA, Archaeal - chemistry; DNA, Archaeal - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Fundamental and applied biological sciences. Psychology; Genes, rRNA; Halalkalicoccus jeotgali; Halobacteriaceae - classification; Halobacteriaceae - genetics; Halobacteriaceae - isolation & purification; Halobacteriaceae - physiology; Hydrogen-Ion Concentration; Korea; Locomotion; Microbiology; Miscellaneous; Molecular Sequence Data; Nucleic Acid Hybridization; Pandalidae - microbiology; Phylogeny; RNA, Archaeal - genetics; RNA, Ribosomal, 16S - genetics; Seafood - microbiology; Sequence Analysis, DNA; Sequence Homology, Nucleic Acid; Sodium Chloride - metabolism; Systematics; Temperature; Korea; Crustacea; Halalkalicoccus; Archaeobacteria; Halophily; Taxonomy; Arthropoda; Bacteria; New species; Decapoda; Invertebrata; Shrimp; Macrura
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.65121-0

1 University of Science & Technology, 52, Eoeun-dong, Daejeon 305-333, Korea 2 Biological Resource Center, KRIBB, Daejeon 305-806, Korea 3 Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju 660-701, Korea Correspondence Jin-Woo Bae baejw{at}kribb.re.kr A novel, extremely halophilic archaeon B3 T was isolated from shrimp-salted seafood. Its morphology, physiology, biochemical features and 16S rRNA gene sequence were characterized. Strain B3 T is non-motile, Gram-variable, requires at least 10 % (w/v) NaCl for growth and grows in the ranges of 21–50 °C and pH 6.5–9.0. The DNA G+C content of strain B3 T was 63.2 mol%. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain B3 T belonged to the genus Halalkalicoccus and was phylogenetically closely related to the type strain Halalkalicoccus tibetensis (98.64 %). However, DNA–DNA hybridization experiments showed 7.0 % relatedness between strain B3 T and a strain of a reference species of the genus Halalkalicoccus . Combined analysis of 16S rRNA gene sequences, DNA–DNA relatedness data, physiological and biochemical tests indicated that the genotypic and phenotypic characteristics differentiate strain B3 T from other Halalkalicoccus species. On the basis of the evidence presented in this report, strain B3 T represents a novel species of the genus Halalkalicoccus , for which the name Halalkalicoccus jeotgali. sp. nov. is proposed. The type strain is B3 T (=KCTC 4019 T =DSM 18796 T =JCM 14584 T =CECT 7217 T ). Abbreviations: PG, phosphatidylglycerol; PGP-Me, phosphatidylglycerol phosphate methyl ester; PGS, phosphatidylglycerol sulfate The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain B3 T is EF077632 .