Phenylalanine and tryptophan stimulate gastrin and somatostatin secretion and H+-K+-ATPase activity in pigs through calcium-sensing receptor

• Published in: General and comparative endocrinology Vol. 267; pp. 1 - 8
• Main Authors: Xian, Yihan, Zhao, Xiuying, Wang, Chao, Kang, Cuicui, Ding, Liren, Zhu, Weiyun, Hang, Suqin
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.10.2018
• Subjects: Animals; Calcium-sensing receptor; Central Nervous System Stimulants; Gastrin; Gastrins - metabolism; H(+)-K(+)-Exchanging ATPase - metabolism; H+-K+-ATPase; Humans; l-Phenylalanine; l-Tryptophan; Phenylalanine - metabolism; Pig stomach; Receptors, Calcium-Sensing - metabolism; Somatostatin; Somatostatin - metabolism; Swine; Tryptophan - metabolism; l-Tryptophan; Pig stomach; Gastrin; H+-K+-ATPase; Calcium-sensing receptor; Somatostatin; l-Phenylalanine; H(+)-K(+)-ATPase
• ISSN: 0016-6480; 1095-6840
• DOI: 10.1016/j.ygcen.2018.05.022

•Phe and Trp stimulate hormone secretion and H+-K+-ATPase activity in pigs.•CaSR mediates hormone secretion and H+-K+-ATPase activity induced by Phe and Trp.•The downstream signaling molecular IP3R is involved in the mediation of CaSR. In rodents and humans, aromatic amino acids increase gut hormone secretion and H+-K+-ATPase activity by modulating calcium-sensing receptor (CaSR). However, the role of CaSR and its related signaling molecules in amino acid-induced gut hormone secretion in swine has not been investigated. Here, we examined whether a CaSR-dependent pathway modulated gastrin and somatostatin (SS) secretion and H+-K+-ATPase activity in pigs. Perfusion of pig stomach tissues in the presence of extracellular 80 mM l-phenylalanine (Phe) or 20 mM l-tryptophan (Trp) and a CaSR agonist cinacalcet triggered gastrin and SS secretion and H+-K+-ATPase activity (P < 0.05) and increased CaSR expression (P < 0.05). This effect of Phe and Trp was dependent on Ca2+ (P < 0.05) and was abolished after treatment with NPS 2143, an inhibitor of CaSR, and 2-aminoethyl diphenyl borinate, an inhibitor of CaSR downstream signaling molecule inositol 1,4,5-triphosphate receptor (IP3R). These findings indicate that Phe and Trp induce Ca2+-dependent gastrin and SS secretion and H+-K+-ATPase activity through CaSR and its downstream signaling molecule IP3R.