Histological and mucin histochemical study of the small intestine of the Persian squirrel (Sciurus anomalus)

• Published in: Anatomical science international Vol. 88; no. 1; pp. 38 - 45
• Main Authors: Tootian, Zahra, Sadeghinezhad, Javad, Sheibani, Mohammad Taghi, Fazelipour, Simin, De Sordi, Nadia, Chiocchetti, Roberto
• Format: Journal Article
• Language: English
• Published: Japan Springer Japan 2013; Springer Nature B.V
• Subjects: Alcian Blue; Anatomy; Animal Anatomy; Animal Physiology; Animals; Cell Biology; Histological Techniques; Histology; Human Physiology; Immunohistochemistry; Intestinal Mucosa - cytology; Intestine, Small - anatomy & histology; Mammals; Medicine; Medicine & Public Health; Morphology; Mucins - metabolism; Muscle, Smooth - cytology; Neurosciences; Original Article; Periodic Acid-Schiff Reaction; Rodents; Rosaniline Dyes; Sciuridae - anatomy & histology; Small intestine; Statistics, Nonparametric; Tissues; Middle East; Histology; Mucin; Persian squirrel; Small intestine; Histochemistry
• ISSN: 1447-6959; 1447-073X
• DOI: 10.1007/s12565-012-0159-5

This article describes the histological and mucin histochemical properties of the small intestine of the Persian squirrel ( Sciurus anomalus ). This species is widely distributed in the Middle East and can be found as a companion animal. The histological studies revealed that the plicae circulares were not visible in the tunica mucosa. The maximum height and width of the villi were observed in the duodenum, which then decreased toward the ileum. The muscularis mucosa was scattered, whereas the tunica submucosa was composed of dense connective tissue. The lymphatic nodules were seen in the submucosa of the distal part of the jejunum and ileum, and Brunner’s glands were embedded in the initial portion of the duodenum. The tunica muscularis was significantly thicker in the ileum, and the circular muscle layer was thicker than the longitudinal muscle layer throughout the entire length of the small intestine. The mucin histochemistry, which was examined using the periodic acid-Schiff (PAS) and alcian blue (AB) (pH 1.0 and 2.5) and also PAS–AB (pH 2.5) and aldehyde fuchsin-AB (pH 2.5) techniques coupled with methylation and saponification reaction for some sections, showed that the small intestine mucous content included both carboxylated and sulfated acidic mucins with few neutral mucins. The results of this study contribute to the knowledge of the histological and histochemical characteristics of the gastrointestinal tracts of exotic mammals and provide data for comparison with other mammals.