Fast and reliable bacterial identification direct from positive blood culture using a new TFA sample preparation protocol and the Vitek® MS system

• Published in: Journal of microbiological methods Vol. 109; pp. 157 - 159
• Main Authors: Monteiro, Jussimara, Inoue, Fernanda Matsiko, Lobo, Ana Paula T., Sugawara, Eduardo K., Boaretti, Fabiana M., Tufik, Sergio
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2015
• Subjects: Bacteremia - diagnosis; Bacteremia - microbiology; Bacterial identification; Bacteriological Techniques - methods; Blood - microbiology; Blood culture; Gram-Negative Bacteria - chemistry; Gram-Negative Bacteria - classification; Gram-Negative Bacteria - isolation & purification; Gram-Negative Bacterial Infections - diagnosis; Gram-Negative Bacterial Infections - microbiology; Gram-Positive Bacteria - chemistry; Gram-Positive Bacteria - classification; Gram-Positive Bacteria - isolation & purification; Gram-Positive Bacterial Infections - diagnosis; Gram-Positive Bacterial Infections - microbiology; Humans; MALDI-TOF; Mass spectrometry; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods; Trifluoroacetic Acid; Vitek MS; Vitek MS; MALDI-TOF; Trifluoroacetic acid; Blood culture; Mass spectrometry; Bacterial identification
• ISSN: 0167-7012; 1872-8359
• DOI: 10.1016/j.mimet.2014.12.009

A simple and reliable protocol using 0.1% trifluoroacetic acid (TFA) was developed to identify bacteria directly from blood cultures on the Vitek® MS system. Results presented a correlation of 99% for Gram-negative bacteria at species level, and 86.3% and 82.3% of Gram-positive bacteria at the genus and species levels, respectively.