Development and Validation of a Method for Assay of the Original Antituberculosis Agent Thiozonide in Plasma for Pharmacokinetic Studies

A method for assaying the antituberculosis drug thiozonide in plasma was developed and validated. Sample preparation consisted of precipitation of proteins with acetonitrile. Assay was by HPLC with a mass-selective detector. The method developed here was validated in terms of the following validatio...

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Bibliographic Details
Published inPharmaceutical chemistry journal Vol. 49; no. 3; pp. 199 - 202
Main Authors Ramenskaya, G. V., Shokhin, I. E., Men’shikova, L. A., Savchenko, A. Yu, Smirnov, V. V., Egorenkov, E. A.
Format Journal Article
LanguageEnglish
Published New York Springer US 01.06.2015
Springer
Subjects
Antitubercular agents
Detection equipment
Medicine
Methods
Methods of Analysis and Process Control
Nitriles
Organic Chemistry
Pharmacology/Toxicology
Pharmacy
Structure of Chemical Compounds
thiozonide
plasma
HPLC-MS
validation
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Summary:A method for assaying the antituberculosis drug thiozonide in plasma was developed and validated. Sample preparation consisted of precipitation of proteins with acetonitrile. Assay was by HPLC with a mass-selective detector. The method developed here was validated in terms of the following validation properties: selectivity, linearity, accuracy, precision, quantitative detection limit, sample transfer, and solution stability. The analytical range of the method was 1 – 1000 ng/ml thiozonide in plasma. This analytical range allows this method to be used for further studies of the pharmacokinetics of the innovative drug thiozonide.
ISSN:0091-150X
1573-9031
DOI:10.1007/s11094-015-1254-4