Characterization and haplotype analysis of 10 novel Y-STR loci in Chinese Han population

• Published in: Forensic science international Vol. 145; no. 1; pp. 47 - 55
• Main Authors: Dai, H.L., Wang, X.D., Li, Y.B., Wu, J., Zhang, J., Zhang, H.J., Dong, J.G., Hou, Y.P.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ireland Ltd 04.10.2004; Elsevier; Elsevier Limited
• Subjects: Biological and medical sciences; China; Chinese Han population; Chromosomes, Human, Y; Deoxyribonucleic acid; DNA; DNA Fingerprinting - methods; DYS454; DYS510; DYS513; DYS520; DYS542; DYS544; DYS552; DYS561; DYS587; DYS593; Ethnic Groups - genetics; Forensic sciences; Frequency distribution; Gene Frequency; General aspects; Genes; Genetic testing; Genetics; Genetics, Population; Haplotypes; Humans; Investigative techniques, diagnostic techniques (general aspects); Male; Medical sciences; Mitochondrial DNA; Multiculturalism & pluralism; Polymerase Chain Reaction; Sex crimes; STR; Tandem Repeat Sequences; Y-specific; Asia; China; DYS510; DYS587; Haplotypes; DYS544; DYS552; DYS454; DYS520; DYS542; DYS561; DYS593; Y-specific; Chinese Han population; DYS513; STR; Human; DYS542: DYS544; Biochemical analysis; Tandemly repeated sequence; Population genetics; Characterization; Microsatellite DNA; Chinese; Population; Haplotype
• ISSN: 0379-0738; 1872-6283
• DOI: 10.1016/j.forsciint.2004.03.009

In this study, we analyzed allelic sequences of 10 novel Y-specific STR loci, DYS454, DYS510, DYS513, DYS520, DYS542, DYS544, DYS552, DYS561, DYS587 and DYS593, surveyed the distribution of haplotypes in a Chinese Han population. Extracted DNA was amplified with PCR, followed by a horizontal non-denaturing polyacrylamide gel electrophoresis with discontinuous buffer system. Purified alleles were sequenced on DNA sequencer (ABI Model 377) to verify the number of motif repeats. The number of alleles observed at each locus ranged from 3 to 8, yielding 102 haplotypes in 103 unrelated males samples. The allele diversity values for each locus ranged from 0.2099 (DYS544) to 0.7523 (DYS552). The haplotype diversity using all these loci was 0.9998. Our study revealed that they were valuable Y-specific markers for forensic applications.