Induction of TNF-α release from human buffy coat cells by Pseudomonas aeruginosa is reduced by lung surfactant protein A

• Published in: FEBS letters Vol. 437; no. 1; pp. 65 - 69
• Main Authors: Hickling, T.P., Sim, R.B., Malhotra, R.
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 16.10.1998
• Subjects: Apoproteins - metabolism; Apoproteins - pharmacology; BC, buffy coat; Collectin; Humans; IL, interleukin; In Vitro Techniques; Innate immunity; LBP, LPS binding protein; Leukocytes - metabolism; Lipopolysaccharide; Lipopolysaccharides - metabolism; Lipopolysaccharides - pharmacology; LPS, lipopolysaccharide; Lung surfactant protein A; P. aeruginosa, Pseudomonas aeruginosa; Pseudomonas aeruginosa - metabolism; Pseudomonas aeruginosa LPS; Pulmonary Surfactant-Associated Proteins; Pulmonary Surfactants - metabolism; Pulmonary Surfactants - pharmacology; SP-A, lung surfactant protein A; TNF-α, tumour necrosis factor alpha; Tumor Necrosis Factor-alpha - metabolism; Tumour necrosis factor-α; Tumour necrosis factor-α; TNF-α, tumour necrosis factor alpha; Innate immunity; Lung surfactant protein A; P. aeruginosa, Pseudomonas aeruginosa; BC, buffy coat; IL, interleukin; Pseudomonas aeruginosa LPS; Lipopolysaccharide; LPS, lipopolysaccharide; LBP, LPS binding protein; Collectin; SP-A, lung surfactant protein A
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/S0014-5793(98)01200-9

Lipopolysaccharide (LPS) induction of TNF-α release is a central event in the pathophysiology of Gram-negative bacterial septicaemia. Lung surfactant protein A (SP-A) mediates pathogen/host cell interactions. Binding of SP-A to Pseudomonas aeruginosa LPS and the effects of SP-A with LPS or whole bacteria on buffy coat cells were investigated. SP-A interacts with P. aeruginosa LPS in a concentration and calcium dependent manner, either through the lipid A portion of LPS or through another lectin/carbohydrate interaction. SP-A decreased TNF-α secretion induced by bacteria or LPS from buffy coat cells, in a concentration dependent manner.