Inherited retinal degeneration: basic FGF induces phagocytic competence in cultured RPE cells from RCS rats

In RCS rats, the retinal pigment epithelium (RPE) is defective in phagocytosis of photoreceptor membranes. We have previously shown reduced expression of basic fibroblast growth factor (bFGF) in the RPE of 7–10-day-old RCS rats. This study using primary RPE cultures from rats of this age demonstrate...

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Bibliographic Details
Published inFEBS letters Vol. 412; no. 1; pp. 21 - 29
Main Authors McLaren, M.J, Inana, G
Format Journal Article
LanguageEnglish
Published England Elsevier B.V 21.07.1997
Subjects
Animals
Basic fibroblast growth factor
Cells, Cultured
Epidermal Growth Factor - pharmacology
Fibroblast Growth Factor 2 - pharmacology
Inherited retinal degeneration
Nerve Growth Factors - pharmacology
Phagocytosis
Pigment Epithelium of Eye - physiopathology
Rats
Rats, Mutant Strains
RCS rat
Retinal Degeneration - genetics
Retinal Degeneration - physiopathology
RPE cell
RPE cell
RCS rat
Basic fibroblast growth factor
Inherited retinal degeneration
Phagocytosis
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Summary:In RCS rats, the retinal pigment epithelium (RPE) is defective in phagocytosis of photoreceptor membranes. We have previously shown reduced expression of basic fibroblast growth factor (bFGF) in the RPE of 7–10-day-old RCS rats. This study using primary RPE cultures from rats of this age demonstrates that the phagocytic defect in the mutant RPE can be overcome by treatment with bFGF, by a mechanism involving gene transcription and that normal RPE phagocytosis, also requiring transcription, is blocked by a bFGF neutralizing antibody. The combined data point to a role for bFGF in the normal mechanism of RPE phagocytosis and the RCS defect.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(97)00566-8