Immunocytochemistry controls using cell culture

• Published in: Diagnostic cytopathology Vol. 17; no. 1; pp. 74 - 79
• Main Authors: Kurtycz, Daniel F.I., Logrono, Roberto, Leopando, Mark, Slattery, Anne, Inhorn, Stanley L.
• Format: Journal Article
• Language: English
• Published: New York Wiley Subscription Services, Inc., A Wiley Company 01.07.1997; Wiley-Liss
• Subjects: Biological and medical sciences; cell culture; cell lines; control material; Humans; immunocytochemistry; Immunohistochemistry; immunologic technique; immunoperoxidase; Investigative techniques, diagnostic techniques (general aspects); Medical sciences; Miscellaneous. Technology; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Tumor Cells, Cultured; Human; Pathology; Cell culture; Immunocytochemistry; Established cell line; Check; Technique
• ISSN: 8755-1039; 1097-0339
• DOI: 10.1002/(SICI)1097-0339(199707)17:1<74::AID-DC17>3.0.CO;2-7

A continuing problem in cytology laboratories is the lack of adequate control material for immunocytochemical testing. Usual control procedures involve testing paraffin‐embedded control materials along with the patient specimens. These control materials are fundamentally unlike cytologic preparations. We have developed a method to make control preparations for immunocytochemical analysis using cultured anaplastic cells with known antigenic features from commercial sources. Cell lines included melanoma, rhabdomyosarcoma, T‐cell leukemia, and squamous‐cell carcinoma. Modified Saccomano and acetone fixation coupled with the cytospin technique enabled good‐quality preparations. Cell lines were tested with antibodies for HMB‐45, actin, leukocyte common antigen (LCA), and cytokeratin, with avidin‐biotin immunoperoxidase and diaminobenzidine (DAB) as chromogens. Our final preparations were easily interpretable with excellent morphologic preservation of cellular detail. Cultured cells provide a superior method for preparing almost unlimited numbers of control slides for immunocytochemistry for laboratories with access to a tissue culture facility. Diagn. Cytopathol. 17:74–79, 1997. © 1997 Wiley‐Liss, Inc.