JNK pathway‐associated phosphatase in acute ischemic stroke patients: Its correlation with T helper cells, clinical properties, and recurrence risk

• Published in: Journal of clinical laboratory analysis Vol. 36; no. 8; pp. e24535 - n/a
• Main Authors: Zhao, Ping, Huo, Huiyong, Li, Juntao, Zhang, Wenchao, Liu, Chao, Jin, Bei, Wang, Huijuan, Wang, Chaohui
• Format: Journal Article
• Language: English
• Published: New York John Wiley & Sons, Inc 01.08.2022; John Wiley and Sons Inc
• Subjects: acute ischemic stroke; Body mass index; CD4+ T cells; Cerebrovascular diseases; Cytokines; Diabetes; disease severity; Enzymes; Etiology; Flow cytometry; Helper cells; Hypertension; Immune response; Immune response (cell-mediated); Inflammation; Interferon; Ischemia; JKAP; Kidney diseases; Lymphocytes T; Medical prognosis; Mortality; Patients; Phosphatase; prognosis; Software; Stroke
• ISSN: 0887-8013; 1098-2825; 1098-2825
• DOI: 10.1002/jcla.24535

Objective JKAP modifies T‐cell immune response and inflammation, also involves in cardia‐cerebrovascular disease etiology. This study intended to explore JKAP's relation with T‐helper 1 (Th1), T‐helper 17 (Th17) cell levels, clinical properties, and recurrence‐free survival (RFS) in acute ischemic stroke (AIS) patients. Methods A total of 155 AIS patients were analyzed. Serum JKAP, interferon‐gamma (IFN‐γ), and interleukin‐17A (IL‐17A) were detected by ELISA; then blood Th1 and Th17 cells were quantified by flow cytometry. Besides, 30 healthy subjects were enrolled as controls to detect JKAP, Th1, and Th17 cells. Results JKAP level was lower (p < 0.001), Th1 cells were not differed (p = 0.068), but Th17 cells were elevated in AIS patients versus controls (p < 0.001). Meanwhile, JKAP was negatively correlated with Th1 cells (p = 0.038), Th17 cells (P<0.001), IFN‐γ (p = 0.002), and IL‐17A (p < 0.001) in AIS patients. JKAP was negatively associated with the National Institutes of Health Stroke Scale (NIHSS) score (p < 0.001), but Th17 cells (p = 0.001), IFN‐γ (p = 0.035), and IL‐17A (p = 0.008) levels were positively associated with NIHSS score. Additionally, accumulating RFS was numerically longer in patients with JKAP Quantile (Q) 4 than patients with JKAP Q1–Q3 (p = 0.068), and numerically better in patients with JKAP Q3–Q4 than patients with JKAP Q1–Q2 (p = 0.069), but without statistical significance. Conclusion JKAP correlates with lower Th1 and Th17 cell percentages as well as milder disease severity. JNK pathway‐associated phosphatase (JKAP) level was lower (p < 0.001), Th1 cell percentage was of no difference (p = 0.068), but Th17 cell percentage was elevated in acute ischemic stroke (AIS) patients than in controls (p < 0.001). Meanwhile, JKAP was negatively correlated with Th1 cells (p = 0.038), Th17 cells (p < 0.001), IFN‐γ (p = 0.002) and IL‐17A (p < 0.001) in AIS patients. JKAP was negatively associated with the National Institutes of Health Stroke Scale (NIHSS) score (p < 0.001), but Th17 cells (p = 0.001), IFN‐γ (p = 0.035) and IL‐17A (p = 0.008) levels were positively associated with NIHSS score. JKAP exhibited a certain value predicting prolonged recurrence‐free survival, but without statistical significance. Conclusively, JKAP correlates with lower Th1 and Th17 cell percentages as well as milder disease severity, which may serve as a biomarker for AIS management.