The effects of mouth rinsing and gargling with mouthwash containing povidone-iodine and hydrogen peroxide on the cycle threshold value of Severe Acute Respiratory Syndrome Coronavirus 2: A randomized controlled trial of asymptomatic and mildly symptomatic patients [version 2; peer review: 2 approved with reservations]

• Published in: F1000 research Vol. 11; p. 1238
• Main Authors: Sulistyani, Lilies Dwi, Julia, Vera, Soeprapto, Andrianto, Swari, Rumartha Putri, Rosmanato, Febriadi, Haryanto, Budi, Cahyarini, Cahyarini, Panjaitan, Rinaldi, Maharani, Diah Ayu
• Format: Journal Article
• Language: English
• Published: England Faculty of 1000 Ltd 2022; F1000 Research Limited; F1000 Research Ltd
• Subjects: Adult; Asymptomatic; Clinical trials; Coronaviruses; COVID-19; COVID-19 - prevention & control; Disease transmission; eng; Female; Humans; Hydrogen Peroxide; Infections; Iodine; Male; Middle Aged; Mouth; mouthwash; Mouthwashes; Nonparametric statistics; Oral cavity; Polymerase chain reaction; Polyvinylpyrrolidone; Povidone; Povidone-iodine; Povidone-Iodine - administration & dosage; Povidone-Iodine - pharmacology; Povidone-Iodine - therapeutic use; Respiration; Respiratory tract; Reverse transcription; RNA polymerase; SARS-CoV-2 - drug effects; severe acute respiratory syndrome coronavirus; Severe acute respiratory syndrome coronavirus 2; Single-Blind Method; Viral Load - drug effects; Young Adult; China; Indonesia; mouthwash; povidone iodine; severe acute respiratory syndrome coronavirus
• ISSN: 2046-1402; 2046-1402
• DOI: 10.12688/f1000research.110843.2

Background Coronavirus disease 2019 can spread rapidly. Surgery in the oral cavity poses a high risk of transmission of severe acute respiratory syndrome coronavirus 2. The American Dental Association and the Centers for Disease Control and Prevention recommend the use of mouthwash containing 1.5% hydrogen peroxide (H 2O 2) or 0.2% povidone iodine (PI) to reduce the viral load in the upper respiratory tract and decrease the risk of transmission. The aim of the present study was to analyze the effect of mouth rinsing and gargling with mouthwash containing 1% PI, 0.5% PI, 3% H 2O 2, or 1.5% H 2O 2 and water on the cycle threshold (CT) value obtained by real-time reverse transcription polymerase chain reaction (RT-PCR). Methods This study is a randomized single blind controlled clinical trial which has been registered in the International Standard Randomized Controlled Trial Number (ISRCTN) registry on the 3 rd February 2022 (Registration number: ISRCTN18356379). In total, 69 subjects recruited from Persahabatan General Hospital who met the inclusion criteria were randomly assigned to one of four treatment groups or the control group. The subjects were instructed to gargle with 15 mL of mouthwash for 30 s in the oral cavity followed by 30 s in the back of the throat, three times per day for 5 days. CT values were collected on postprocedural days 1, 3, and 5. Results The results of the Friedman test significantly differed among the groups (n=15). The CT values increased from baseline (day 0) to postprocedural days 1, 3, and 5. Conclusions Mouth rinsing and gargling with mouthwash containing 1% PI, 0.5% PI, 3% H 2O 2, or 1.5% H 2O 2 and water increased the CT value.