Combined photodynamic and photothermal induced injury enhances damage to in vivo model blood vessels
Background and Objectives The degree of port wine stain (PWS) blanching following pulsed dye laser (PDL) therapy remains variable and unpredictable. Because of the limitations of current PDL therapy, alternative treatment approaches should be explored. The objective was to evaluate a novel methodolo...
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Published in | Lasers in surgery and medicine Vol. 34; no. 5; pp. 407 - 413 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Hoboken
Wiley Subscription Services, Inc., A Wiley Company
01.06.2004
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Subjects | |
Online Access | Get full text |
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Summary: | Background and Objectives
The degree of port wine stain (PWS) blanching following pulsed dye laser (PDL) therapy remains variable and unpredictable. Because of the limitations of current PDL therapy, alternative treatment approaches should be explored. The objective was to evaluate a novel methodology for selective vascular damage, combined photodynamic (PDT) and photothermal (PDL) treatment, using the in vivo chick chorioallantoic membrane (CAM) model.
Study Design/Materials and Methods
Thirty microliters of benzoporphyrin derivative monoacid ring A (BPD) solution was administered intraperitoneally into chick embryos at day 12 of development. Study groups were: (1) control (no BPD, no light); (2) BPD alone; (3) continuous wave irradiation (CW) alone (576 nm, 60 mW/cm2, 125 seconds); (4) CW + PDL; (5) BPD+PDL; (6) PDT (BPD+CW); (7) PDL alone (585 nm, 4 J/cm2); and (8) PDT+PDL (BPD + CW followed immediately by PDL). Vessels were videotaped prior to, and at 1 hour post‐intervention and then assessed for damage based on the following scale: 0, no damage; 1, coagulation; 1.5, vasoconstriction; 2.0, coagulation+vasoconstriction; 2.5, angiostasis; 3.0, hemorrhage. Damage scores were weighted by vessel “order.”
Results
PDT + PDL resulted in significantly (P < 0.01) more severe vascular damage than was observed in any other study group: 127% more than PDT, 47% more than PDL alone.
Conclusions
PDT + PDL is a novel and promising approach for selective vascular damage and may offer a more effective method for treatment of PWS and other vascular skin lesions. Lasers Surg. Med. 34:407–413, 2004. © 2004 Wiley‐Liss, Inc. |
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Bibliography: | Beckman Laser Institute Endowment ark:/67375/WNG-91WDJJTX-3 istex:749E0E2B9EEA770E5D4CA111D40E3736BC7C06CC National Institutes of Health (to JSN) - No. GM-62177; No. EB002495; No. AR-47551 Dermatology Foundation (to KMK) Air Force Office of Scientific Research National Science Foundation (to TS and AS) ArticleID:LSM20041 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 0196-8092 1096-9101 |
DOI: | 10.1002/lsm.20041 |