Capillary electrochromatography/mass spectrometry - a comparison of the sensitivity of nanospray and microspray ionization techniques
Capillary electrochromatography/mass spectrometry (CEC/MS) has so far only been performed using electrospray or microspray ionization. CEC has, to date, not been reported coupled online to nanospray ionization, due to practical difficulties in coupling the CEC column to the nanospray emitter. Howeve...
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Published in | Rapid communications in mass spectrometry Vol. 12; no. 17; pp. 1143 - 1149 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Chichester, UK
John Wiley & Sons, Ltd
15.09.1998
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Online Access | Get full text |
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Summary: | Capillary electrochromatography/mass spectrometry (CEC/MS) has so far only been performed using electrospray or microspray ionization. CEC has, to date, not been reported coupled online to nanospray ionization, due to practical difficulties in coupling the CEC column to the nanospray emitter. However this combination is ideally suited, as the flow rates for CEC (100nL/min, approximately) are directly compatible with larger orifice (10μm) nanospray emitters.
A CEC unit has been designed and engineered in‐house, to enable the use of short columns and high field strengths, facilitating fast electro‐osmotic flow and short retention times. This device was initially coupled via a 50 μm × 37 cm C18/SCX column, and then by a C6/SCX 50 μm × 45 cm column, to microspray and nanospray interfaces, also designed and built in‐house. CEC/microspray‐MS and CEC/nanospray‐MS were successfully evaluated on an ion‐trap mass spectrometer using five corticosteriods with the addition of thiourea as a flow marker. Limits of detection (S/N = 3) were found to be 500 fg (1–2 mol) for CEC/microspray‐MS and initial studies using the nanospray interface have predicted detection limits in the region of 50 fg (∼100 amol). © 1998 John Wiley & Sons, Ltd. |
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Bibliography: | istex:6179669CDA1A5C57874742E67D8ABB65C349634E British Mass Spectrometry Society ArticleID:RCM280 ark:/67375/WNG-594TBBWM-4 EPSRC Chemistry Programme in Analytical Science - No. GR/L 35942 Finnigan Corporation, San Jose, USA Glaxo Wellcome, Stevenage, UK |
ISSN: | 0951-4198 1097-0231 |
DOI: | 10.1002/(SICI)1097-0231(19980915)12:17<1143::AID-RCM280>3.0.CO;2-3 |