Search for Protein Adhesin Gene in Bifidobacterium Longum Genome Using Surface Phage Display Technology

• Published in: Bulletin of experimental biology and medicine Vol. 146; no. 6; pp. 782 - 785
• Main Authors: Shkoporov, A. N, Khokhlova, E. V, Kafarskaia, L. I, Pavlov, K. A, Smeianov, V. V, Steele, J. L, Efimov, B. A
• Format: Journal Article
• Language: English; Russian
• Published: Boston Boston : Springer US 01.12.2008; Springer US; Springer Nature B.V
• Subjects: adhesin; Adhesins, Bacterial - chemistry; Adhesins, Bacterial - genetics; adhesion; Amino Acid Sequence; bifidobacteria; Bifidobacterium - genetics; Bifidobacterium longum; Biomedical and Life Sciences; Biomedicine; Cell Biology; Genetics; Genome, Bacterial - genetics; HT-29; HT29 Cells; Humans; Internal Medicine; Laboratory Medicine; Pathology; Peptide Library; phage display; Sequence Alignment; bifidobacteria; adhesin; adhesion; HT-29; phage display
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-009-0423-4

A fragment of the nucleotide sequence encoding polypeptide binding to HT-29 epithelial cells was cloned from VMKB44 Bifidobacterium longum genome library using surface phage display technology. Sequencing of this polypeptide consisting of 26 amino acid residues showed that it is an extracellular fragment of a large BL0155 transmembrane protein belonging to the ABC transport protein superfamily. The genes encoding homologues of this protein were detected in genomes of not only bifidobacteria of different species, but also in many other enteric commencals and pathogens.