Immunization with Staphylococcus aureus Lysate Incorporated into Microspheres

• Published in: Journal of dairy science Vol. 84; no. 8; pp. 1791 - 1799
• Main Authors: O'Brien, C. N, Guidry, A. J, Douglass, L. W, Westhoff, D. C
• Format: Journal Article
• Language: English
• Published: Savoy, IL Am Dairy Sci Assoc 01.08.2001; American Dairy Science Association
• Subjects: Animal bacterial diseases; Animal productions; Animals; Antibodies, Bacterial - biosynthesis; Antigens, Bacterial - immunology; Bacterial diseases; Bacterial Vaccines - administration & dosage; Bacterial Vaccines - immunology; Biological and medical sciences; Cattle; Cell Wall - immunology; Enzyme-Linked Immunosorbent Assay - veterinary; Female; Flow Cytometry - veterinary; Fundamental and applied biological sciences. Psychology; Infectious diseases; Injections, Intralymphatic - veterinary; Injections, Intramuscular - veterinary; Mastitis, Bovine - prevention & control; Medical sciences; Microspheres; Opsonin Proteins; Particle Size; Phagocytosis; Polysaccharides, Bacterial - immunology; Staphylococcus aureus - immunology; Terrestrial animal productions; Vaccination - methods; Vaccination - veterinary; Vertebrates; Encapsulation; Mastitis; Farming animal; Lysate; Microsphere; Ruminant animal; Vaccination; Dairy cattle; Experimental study; Veterinary medicine; Milk production; Infection; Mammary gland diseases; Vertebrata; Mammalia; Pathogenic; Bacteria; Micrococcales; Micrococcaceae; Artiodactyla; Neutrophil; Ungulata; Phagocytosis; Staphylococcus aureus
• ISSN: 0022-0302; 1525-3198
• DOI: 10.3168/jds.S0022-0302(01)74617-6

Antibiotics are of limited value against Staphylococcus aureus due to development of resistant strains, scar tissue formation, and blockage of ducts due to inflammation. Though macrophages are the predominant cell type in the mammary gland, they are primarily scavenger cells and are not effective against bacteria entering the gland. Neutrophil phagocytosis is the bovine's primary defense against S. aureus mastitis. Attempts to develop vaccines that enhance neutrophil phagocytosis by stimulating production of opsonizing antibodies to S. aureus have met with limited success because of the low immunogenicity of the exopolysaccharide capsule surrounding S. aureus. Staphylococcus aureus can also adhere to and penetrate epithelial tissue. This study was conducted to determine whether lysates of S. aureus encapsulated in biodegradable microspheres would increase the production of opsonizing antibodies to capsule and block adherence. Four groups of four cows each were injected with 1 ml of the respective treatment in the area of the supramammary lymph node and 1 ml in the hip muscle. The treatments were: lysate in NaCl, lysate in Freund's incomplete adjuvant (FICA), lysate in microspheres in NaCl, and lysate in microspheres in FICA. Antigen in microspheres produced a similar antibody response to antigen emulsified in FICA, but to a lesser magnitude. Antigen in microspheres produced antibodies that were more opsonic for neutrophils at 20 and 52 wk postimmunization and inhibited S. aureus adherence to mammary epithelium. Ability to control antigen release and presentation, and the benefit of a single injection for long-term immunity using microspheres warrants additional studies.