Mannanase Transfer into Hexane and Xylene by Liquid-Liquid Extraction

• Published in: Applied biochemistry and biotechnology Vol. 160; no. 4; pp. 1124 - 1129
• Main Authors: Shipovskov, Stepan, Kragh, Karsten M, Laursen, Brian S, Poulsen, Charlotte H, Besenbacher, Flemming, Sutherland, Duncan S
• Format: Journal Article
• Language: English
• Published: New York New York : Humana Press Inc 01.02.2010; Humana Press Inc; Springer; Springer Nature B.V
• Subjects: beta-mannosidase; Biochemistry; Bioconversions. Hemisynthesis; Biological and medical sciences; Biotechnology; Catalysis; catalysts; Chemical Fractionation - methods; Chemistry; Chemistry and Materials Science; Enzymes; extraction; Extraction processes; Fundamental and applied biological sciences. Psychology; glycosidases; hexane; Hexanes - chemistry; Hydrocarbons; locust bean gum; Mannosidases - chemistry; Mannosidases - isolation & purification; Mannosidases - metabolism; Methods. Procedures. Technologies; Organic solvents; Solubility; Solvents; Succinates - chemistry; surfactants; Xylene; Xylenes - chemistry; mannanase; Organic solvent; Biocatalysis; Xylene; Endo-1,4-β; Liquid–liquid extraction; Hexane; Enzyme; Glycosylases; Mannan endo-1,4-β-mannosidase; β-Mannosidase; Liquid―liquid extraction; Glycosidases; Endo-1,4-β-D-mannanase; Transfer; Hydrolases; Extraction
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-009-8661-5

The formation of noncovalent complexes between glycosidase, endo-1,4-β-d-mannanase, and ionic surfactant di(2-ethylhexyl) sodium sulfosuccinate (AOT) was shown to promote protein transfer into organic solvents such as xylene and hexane. It was found that mannanase can be solubilized in hexane and in xylene with concentration at least 2.5 and 2.0 mg/ml, respectively. The catalytic activity of the enzyme in hexane spontaneously increases with the concentration of AOT and is about 10% of the activity in aqueous system. In xylene, a catalytic activity higher than that in bulk aqueous conditions was found for the samples containing 0.1-0.3 mg/ml of mannanase, while for the samples with a higher concentration of enzyme, the activity was hardly detected.