Gene silencing of Dim-1, a member of the disorganized muscle family, in Haemonchus contortus

• Published in: Molecular and biochemical parasitology Vol. 211; pp. 71 - 74
• Main Authors: Arenal, Amilcar, Díaz, Arnielis, Sprenger, Lew Kan, Buzatti, Andreia, Fernandes, Maria Angela Machado, dos Santos, Jeannie Nascimento, Molento, Marcelo
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.01.2017
• Subjects: Animals; Gene Silencing; Haemonchiasis - parasitology; Haemonchus - genetics; Haemonchus - growth & development; Helminth Proteins - genetics; Larva; Larval development; Larval migration; RNA Interference; Sheep; Larval development; RNA interference; Larval migration
• ISSN: 0166-6851; 1872-9428
• DOI: 10.1016/j.molbiopara.2016.10.004

[Display omitted] •Specific silencing of the Dim-1 gene of H. contortus.•Dim-1 RNA interference (RNAi) results in arrested H. contortus larval development.•Dim-1 RNAi results in loss of migration capacity of H. contortus larvae. RNA interference has been widely used in parasites. In Haemonchus contortus, reproducible silencing has been reported; however, in this species an altered phenotype has been observed for only a few genes silenced by RNA interference. The aim of this work was to evaluate the effect of silencing Dim-1, a member of the disorganized muscle family on third stage larvae (L3) of H. contortus. Effective silencing of Dim-1 in L3 led to reduced L3 migration and slowed larval development from L3 to early L4. To our knowledge, this is the first report to demonstrate Dim-1 silencing affecting phenotypic characteristics of this parasite.