Actinidia arguta Sprout as a Natural Antioxidant: Ameliorating Effect on Lipopolysaccharide-Induced Cognitive Impairment

• Published in: Journal of microbiology and biotechnology Vol. 31; no. 1; pp. 51 - 62
• Main Authors: Kang, Jeong Eun, Park, Seon Kyeong, Kang, Jin Yong, Kim, Jong Min, Kwon, Bong Seok, Park, Sang Hyun, Lee, Chang Jun, Yoo, Seul Ki, Heo, Ho Jin
• Format: Journal Article
• Language: English
• Published: Korea (South) Korean Society for Microbiology and Biotechnology 28.01.2021; 한국미생물·생명공학회
• Subjects: Acetylcholinesterase - metabolism; Actinidia - chemistry; Animals; Anti-Inflammatory Agents - pharmacology; Antioxidants - pharmacology; Brain - metabolism; Cognitive Dysfunction - chemically induced; Cognitive Dysfunction - drug therapy; HT29 Cells; Humans; Inflammation - drug therapy; Interleukin-6; Limosilactobacillus reuteri; Lipopolysaccharides - adverse effects; Male; Mice; Neuroprotective Agents - pharmacology; NF-kappa B - metabolism; Phytochemicals - pharmacology; Plant Extracts - pharmacology; Research article; Superoxide Dismutase; 생물학; anti-inflammation; Actinidia arguta sprout; lipopolysaccharide; antioxidants; neuroprotection
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.2009.09012

Here, we investigated the prebiotic and antioxidant effects of sprout water extract (AASWE) on lipopolysaccharide (LPS)-induced cognitive deficit mice. AASWE increased viable cell count, titratable acidity, and acetic acid production in strain and showed a cytoprotective effect on LPS-induced inflammation in HT-29 cells. We assessed the behavior of LPSinduced cognitive deficit mice using Y-maze, passive avoidance and Morris water maze tests and found that administration of AASWE significantly improved learning and memory function. The AASWE group showed antioxidant activity through downregulation of malondialdehyde levels and upregulation of superoxide dismutase levels in brain tissue. In addition, the AASWE group exhibited activation of the cholinergic system with decreased acetylcholinesterase activity in brain tissue. Furthermore, AASWE effectively downregulated inflammatory mediators such as phosphorylated- JNK, phosphorylated-NF-κB, TNF-α and interleukin-6. The major bioactive compounds of AASWE were identified as quercetin-3- -arabinopyranosyl(1→2)-rhamnopyranosyl(1→6)-glucopyranose, quercetin-3- -apiosyl(1→2)-galactoside, rutin, and 3-caffeoylquinic acid. Based on these results, we suggest that AASWE not only increases the growth of beneficial bacteria in the intestines, but also shows an ameliorating effect on LPS-induced cognitive impairment.