Duck PIAS2 negatively regulates RIG-I mediated IFN-β production by interacting with IRF7

• Published in: Developmental and comparative immunology Vol. 108; p. 103664
• Main Authors: Zu, Shaopo, Xue, Qian, He, Zhuoliang, Shi, Chenxi, Wu, Wenbo, Zhang, Junsheng, Li, Weiqiang, Huang, Jianni, Jiao, Peirong, Liao, Ming
• Format: Journal Article
• Language: English
• Published: United States Elsevier Ltd 01.07.2020; Elsevier Science Ltd
• Subjects: Activation; Aquatic birds; Duck; IFN-β; Immune response; Immune system; Innate immunity; Interferon; Interferon regulatory factor; Interferon regulatory factor 3; Interferon regulatory factor 7; IRF7; NF-κB protein; PIAS; Proteins; Signal transduction; Transcription factors; Ubiquitin-protein ligase; Viruses; β-Interferon; IRF7; Duck; IFN-β; PIAS
• ISSN: 0145-305X; 1879-0089
• DOI: 10.1016/j.dci.2020.103664

The protein inhibitor of activated STAT (PIAS) proteins are important signal transduction modulator family and regulate the innate immune signaling pathway induced by certain transcription factors, including NF-κB, IRF3, and JAK/STAT. The PIAS protein mechanism that regulates innate immune response in mammals has been well described in the literature; however, whether the PIAS gene exists in ducks as well as the role of PIAS in duck IFN-β expression is still unclear. Here, we cloned duck PIAS (duPIAS), finding PIAS2 could repress IFN-β production. DuPIAS2 contains SAP-PINIT-RLD-S/T characteristic domains, and its overexpression could inhibit virus-induced IFN-β promoter activation. Moreover, duPIAS2 interacts with duck interferon regulatory factor 7 (IRF7) and inhibits IFN-β promoter activation induced by duck IRF7. Additionally, its inhibitory function does not rely on its SUMO E3 ligase activity but rather its C-terminal portion. The above results demonstrate that duPIAS2 is a repressor of IFN-β production induced by duck IRF7. •The first avian PIAS (DK-PIAS) family was identified from duck cells.•DK-PIAS2 represses DK-RIG-Ⅰ mediated IFN-β signaling.•DK-PIAS2 interacts with DK-IRF7 and inhibits IRF7-induced IFN-β production.