Effluviibacter roseus gen. nov., sp. nov., isolated from muddy water, belonging to the family 'Flexibacteraceae'

• Published in: International journal of systematic and evolutionary microbiology Vol. 56; no. 7; pp. 1703 - 1707
• Main Authors: Suresh, K, Mayilraj, S, Chakrabarti, T
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.07.2006; Society for General Microbiology
• Subjects: Aerobiosis; Amino Acids - metabolism; Bacterial Typing Techniques; Bacteriology; Base Composition; Biological and medical sciences; Carbohydrate Metabolism; Cytophagaceae - classification; Cytophagaceae - cytology; Cytophagaceae - isolation & purification; Cytophagaceae - physiology; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Effluviibacter roseus; Fatty Acids - analysis; Fatty Acids - chemistry; Flexibacteraceae; Freshwater; Fundamental and applied biological sciences. Psychology; Genes, rRNA; Gentian Violet; Microbiology; Molecular Sequence Data; Phenazines; Phylogeny; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Sequence Homology, Nucleic Acid; Systematics; Water Microbiology; Bacteria; New genus; New species; Taxonomy
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.64144-0

Microbial Type Culture Collection and Gene Bank (MTCC), Institute of Microbial Technology, Sector 39A, Chandigarh – 160 036, India Correspondence T. Chakrabarti tapan{at}imtech.res.in A Gram-negative bacterial isolate (designated SRC-1 T ) was isolated from an occasional drainage system and characterized by a polyphasic approach to determine its taxonomic position. Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain SRC-1 T with the family ‘ Flexibacteraceae ’ of the phylum Bacteroidetes . It showed greatest sequence similarity to Pontibacter actiniarum KMM 6156 T (95.5 %) followed by Adhaeribacter aquaticus MBRG1.5 T (89.0 %) and Hymenobacter roseosalivarius DSM 11622 T (88.9 %), but it differed from these micro-organisms in many phenotypic characteristics. Strain SRC-1 T was an obligate aerobe and its cells were non-motile, irregular rods. The major fatty acids included mainly unsaturated and hydroxy fatty acids, including 17 : 1 iso I/anteiso B (36.7 %), 15 : 0 iso (15.8 %) and 17 : 0 iso 3-OH (10.3 %), and the DNA G+C content was 59.5 mol%. From the phenotypic and genotypic analyses it was clear that strain SRC-1 T was quite different from members other genera in the family ‘ Flexibacteraceae ’. Therefore we conclude that strain SRC-1 T represents a novel genus, for which the name Effluviibacter gen. nov., containing a single species Effluviibacter roseus sp. nov., is proposed. The type species of the genus is Effluviibacter roseus , the type strain of which is strain SRC-1 T (=MTCC 7260 T =DSM 17521 T ). The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain SRC-1 T is AM049256 .