The effect of freezing on the potassium ferricyanide-potassium cyanide reagent used in the cyanmethemoglobin procedure for hemoglobin determination

The instability on freezing of solutions containing both potassium ferricyanide and potassium cyanide is pointed out by spectrophotometry and by testing for ferricyanide and ferrocyanide ions. The consequences from use of solutions which have been subjected to freezing conditions are demonstrated. O...

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Published inClinica chimica acta Vol. 9; no. 6; pp. 581 - 584
Main Authors Weatherburn, M.W., Logan, J.E.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.06.1964
Subjects
Cyanides
Ferricyanides
Ferrocyanides
Freezing
Hematologic Tests
Hemoglobinometry
Indicators and Reagents
Methemoglobin
Old Medline
Potassium
Potassium Cyanide
Temperature
FERROCYANIDES
TEMPERATURE
METHEMOGLOBIN
POTASSIUM
INDICATORS AND REAGENTS
CYANIDES
HEMOGLOBINOMETRY
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Summary:The instability on freezing of solutions containing both potassium ferricyanide and potassium cyanide is pointed out by spectrophotometry and by testing for ferricyanide and ferrocyanide ions. The consequences from use of solutions which have been subjected to freezing conditions are demonstrated. On testing commercial preparations of diluent prepared for the cyanmethemoglobin method it was found that three out of ten were unsatisfactory on arrival, one from transportation in cold weather, two from other causes. The possibility of deterioration of other products due to oxidation-reduction reactions at low temperature is mentioned. The responsibility of manufacturers for adequate control measures before the distribution of reagents, especially those in solution, is stressed.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0009-8981
1873-3492
DOI:10.1016/0009-8981(64)90125-1