Expression of Circulating MicroRNA-141 in Epithelial Ovarian Cancer

Epithelial ovarian cancer (EOC) is a lethal disease due to late diagnosis and lack of effective screening methods. MicroRNA (miR/miRNA) plays an important role in ovarian carcinogenesis and may serve as a non-invasive biomarker for EOC. This study aimed to assess miR-141 expression in the blood plas...

Full description

Saved in:
Bibliographic Details
Published inThe Malaysian journal of medical sciences Vol. 27; no. 6; pp. 27 - 38
Main Authors Fitriawan, Akbar Satria, Kartika, Aprilia Indra, Chasanah, Siti Nur, Aryandono, Teguh, Haryana, Sofia Mubarika
Format Journal Article
LanguageEnglish
Published Malaysia Universiti Sains Malaysia Press 01.12.2020
Penerbit Universiti Sains Malaysia
Subjects
Biomarkers
Colorectal cancer
Disease
Fallopian tubes
Gene expression
Medical prognosis
Metastasis
MicroRNAs
Mortality
Original
Ovarian cancer
Plasma
Proteins
Standard deviation
microRNA
epithelial ovarian cancer
miR-141
biomarker
plasma
Online AccessGet full text

Cover

More Information
Summary:Epithelial ovarian cancer (EOC) is a lethal disease due to late diagnosis and lack of effective screening methods. MicroRNA (miR/miRNA) plays an important role in ovarian carcinogenesis and may serve as a non-invasive biomarker for EOC. This study aimed to assess miR-141 expression in the blood plasma of patients with EOC and healthy subjects and determine its association with the clinical stage of EOC. This cross-sectional study used blood plasma from 30 newly diagnosed untreated patients with EOC and 25 healthy subjects. The mean age was 47.73 (SD = 10.29) years for EOC and 44.48 (SD = 16.14) years for healthy subject. The total RNA was isolated from blood plasma and reversed transcribed to obtain cDNA. The expression of miR-141 was measured by real-time quantitative polymerase chain reaction (qRT-PCR), and calculated using 2 methods. The data were analysed using Mann-Whitney test. The expression of miR-141 was upregulated 8.41 fold in the blood plasma of EOC patients compared to healthy controls ( < 0.001). Expression of miR-141 in the advanced stage was upregulated 4.2 fold compared to the early stage ( < 0.001). The miR-141 was upregulated in the blood plasma of EOC and associated with an advanced stage of disease, suggesting it has potential as a biomarker for EOC detection.
ISSN:1394-195X
2180-4303
DOI:10.21315/mjms2020.27.6.4