Modulation of macrophage cytokine production by conjugated linoleic acids is influenced by the dietary n-6:n-3 fatty acid ratio

• Published in: The Journal of nutritional biochemistry Vol. 9; no. 5; pp. 258 - 266
• Main Authors: Turek, John J., Li, Yong, Schoenlein, Ingrid A., Allen, Ken G.D., Watkins, Bruce A.
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.05.1998; Elsevier Science
• Subjects: ACEITE DE CARTAMO; ACEITE DE SOJA; ACEITES DE PESCADO; ACIDE GRAS; ACIDE GRAS POLYINSATURE; ACIDE LINOLEIQUE; ACIDO LINOLEICO; ACIDOS GRASOS; ACIDOS GRASOS POLIINSATURADOS; ACTIVITY; ANIMAL MODELS; BAZO; Biological and medical sciences; BIOSINTESIS; BIOSYNTHESE; BIOSYNTHESIS; CITOQUINAS; conjugated linoleic acids; CORPS GRAS; CYTOKINE; CYTOKINES; DIET; DIETA; ESTIMULO; EXPERIMENTACION; EXPERIMENTATION; FATS; FATTY ACIDS; Feeding. Feeding behavior; FISH OILS; FOIE; Fundamental and applied biological sciences. Psychology; GRASAS; HIGADO; HUILE DE CARTHAME; HUILE DE POISSON; HUILE DE SOJA; IL-1; IL-6, TNF; INTERLEUKINE; INTERLEUKINS; INTERLEUQUINAS; ISOMERS; LINOLEIC ACID; LIPID METABOLISM; LIPOPOLISACARIDOS; LIPOPOLYSACCHARIDE; LIPOPOLYSACCHARIDES; LIVER; MACROFAGOS; MACROPHAGE; MACROPHAGES; MENHADEN; METABOLISME DES LIPIDES; METABOLISMO DE LIPIDOS; MODELE ANIMAL; MODELOS ANIMALES; OMEGA-3 FATTY ACIDS; OMEGA-6 FATTY ACIDS; PGE 2; POLYUNSATURATED FATTY ACIDS; PROSTAGLANDINAS; PROSTAGLANDINE; PROSTAGLANDINS; RAT; RATA; RATE; RATS; REGIME ALIMENTAIRE; SAFFLOWER OIL; SOYBEAN OIL; SPLEEN; STIMULI; STIMULUS; TUMOR NECROSIS FACTOR; Vertebrates: anatomy and physiology, studies on body, several organs or systems; conjugated linoleic acids; PGE 2; macrophage; IL-6, TNF; IL-1; Vertebrata; Mammalia; n-6 fatty acid; Rat; Diet; Rodentia; Cytokine; Lipids; n-3 fatty acid; Nutritional status; Feeding; Linoleic acid
• ISSN: 0955-2863; 1873-4847
• DOI: 10.1016/S0955-2863(98)00014-X

Conjugated linoleic acids (CLA) are a mixture of 18:2 isomeric fatty acids that have conjugated double bonds. We undertook this study to determine if CLA altered spleen fatty acid composition, macrophage cytokine production, and liver prostaglandin E 2 (PGE 2) biosynthesis in the presence of varying ratios of (n-6) and (n-3) polyunsaturated fatty acids (PUFA). Groups of six rats were fed diets (AIN-93G) containing soybean oil (SBO) or menhaden oil and safflower oil (MSO), with CLA (10 g/kg) or without CLA. Spleens of rats consuming CLA contained the t9,c11 and t10,c12 isomers of CLA and a lower content of 18:1 fatty acids. CLA significantly reduced basal and lipopolysaccharide (LPS) induced levels of interleukin-6 (IL-6) by resident peritoneal macrophages in rats given SBO. CLA also reduced basal levels of tumor necrosis factor (TNF) production in both diets, but had no effect on LPS-induced levels of TNF. CLA had no effect on IL-1 production, but macrophage IL-1 production was increased in MSO fed rats compared with SBO fed rats. Rats given MSO had reduced PGE 2 production in spleen compared with SBO fed rats. In SBO fed rats, CLA caused some reduction in PGE 2, but the effect was not significant. The effect of CLA on PGE 2 production may be dependent on the type of dietary fat consumed and the organ being studied.