Identification of a necessary element for Toxoplasma gondii SAG1 gene expression
SAG1 codes for the stage-specific major surface antigen P30 of Toxoplasma gondii ( T. gondii) tachyzoites. Six tandemly repeated, conserved 27 bp cassettes in the region from −231 to −70 bp were previously confirmed to be essential for high-level expression of SAG1 and serve as a positioning element...
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Published in | Experimental parasitology Vol. 116; no. 2; pp. 175 - 181 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
San Diego, CA
Elsevier Inc
01.06.2007
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | SAG1 codes for the stage-specific major surface antigen P30 of
Toxoplasma gondii (
T. gondii) tachyzoites. Six tandemly repeated, conserved 27
bp cassettes in the region from −231 to −70
bp were previously confirmed to be essential for high-level expression of
SAG1 and serve as a positioning element directing the initiation of transcription. We demonstrate here that an element located between +19 and +28
bp is necessary for
SAG1 gene expression by using deletion mutagenesis analysis and electrophoresis mobility shift assay (
EMSA). This will provide an insight into the regulatory mechanisms of
SAG1 gene expression. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0014-4894 1090-2449 |
DOI: | 10.1016/j.exppara.2006.11.007 |