Sweat Testing for the Detection of Methylone after Controlled Administrations in Humans

The aim of this study was to determine the excretion of methylone and its metabolites in sweat following the ingestion of increasing controlled doses of 50, 100, 150 and 200 mg of methylone to twelve healthy volunteers involved in a clinical trial. Methylone and its metabolites 4-hydroxy-3-methoxy-N...

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Published inInternational journal of molecular sciences Vol. 24; no. 8; p. 7395
Main Authors Di Giorgi, Alessandro, Sprega, Giorgia, Poyatos, Lourdes, Papaseit, Esther, Pérez-Mañá, Clara, Di Trana, Annagiulia, Varì, Maria Rosaria, Busardò, Francesco Paolo, Pichini, Simona, Zaami, Simona, Lo Faro, Alfredo Fabrizio, Farré, Magí
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 17.04.2023
MDPI
Subjects
Chromatography, Liquid - methods
Communication
Humans
Mass Spectrometry
Metabolites
Methamphetamine - metabolism
Sweat - chemistry
Germany
pharmacokinetics
sweat
LC-MS/MS
methylone
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Summary:The aim of this study was to determine the excretion of methylone and its metabolites in sweat following the ingestion of increasing controlled doses of 50, 100, 150 and 200 mg of methylone to twelve healthy volunteers involved in a clinical trial. Methylone and its metabolites 4-hydroxy-3-methoxy-N-methylcathinone (HMMC) and 3,4-methylenedioxycathinone (MDC) were analyzed in sweat patches by liquid chromatography–tandem mass spectrometry. Methylone and MDC were detected in sweat at 2 h and reached their highest accumulation (Cmax) at 24 h after the administration of 50, 100, 150 and 200 mg doses. In contrast, HMMC was not detectable at any time interval after each dose. Sweat proved to be a suitable matrix for methylone and its metabolites’ determination in clinical and toxicological studies, providing a concentration that reveals recent drug consumption.
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These authors contributed equally to this work.
These authors also contributed equally to this work.
ISSN:1422-0067
1422-0067
DOI:10.3390/ijms24087395