Ricinosomes provide an early indicator of suspensor and endosperm cells destined to die during late seed development in quinoa (Chenopodium quinoa)

• Published in: Annals of botany Vol. 112; no. 7; pp. 1253 - 1262
• Main Authors: López-Fernández, M. P, Maldonado, S
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.11.2013
• Subjects: Apoptosis; castor beans; Cell Death; Cell nucleus; Cell Nucleus - metabolism; Cell walls; Chenopodium quinoa; Chenopodium quinoa - cytology; Chenopodium quinoa - enzymology; Chenopodium quinoa - growth & development; Chenopodium quinoa - ultrastructure; cysteine; Cysteine Endopeptidases - metabolism; Cytoplasm; cytosol; DNA; DNA Fragmentation; Embryos; Endosperm; Endosperm - cytology; Endosperm - growth & development; Endosperm - ultrastructure; Flow Cytometry; germination; light microscopy; lipids; Mother cells; Organelles - enzymology; Organelles - metabolism; Organelles - ultrastructure; Original; Plant cells; Quinoa; Ricinus communis; Seed development; seeds; Subcellular Fractions - metabolism; transmission electron microscopy; vacuoles; Western blotting; Cys-EP in situ immunolocalization; Cys-EP western blot; cysteine endopeptidase; ricinosomes; Cys-EP; Amaranthaceae; endosperm; Chenopodiaceae; PCD; TUNEL; micropylar endosperm; suspensor; programmed cell death; Chenopodium quinoa
• ISSN: 0305-7364; 1095-8290
• DOI: 10.1093/aob/mct184

Background and AimsIn mature quinoa (Chenopodium quinoa) seeds, the lasting endosperm forms a micropylar cone covering the radicle. The suspensor cells lie within the centre of the cone. During the final stage of seed development, the cells of the lasting endosperm accumulate protein and lipids while the rest are crushed and disintegrated. Both the suspensor and endosperm die progressively from the innermost layers surrounding the embryo and extending towards the nucellar tissue. Ricinosomes are endoplasmic reticulum-derived organelles that accumulate both the pro-form and the mature form of cysteine endopeptidase (Cys-EP), first identified in castor bean (Ricinus communis) endosperm during germination. This study sought to identify associations between the presence of ricinosomes and programmed cell death (PCD) hallmarks in suspensor and endosperm cells predestined to die during quinoa seed development.MethodsA structural study using light microscopy and transmission electron microscopy was performed. To detect the presence of Cys-EP, both western blot and in situ immunolocalization assays were carried out using anti-R. communis Cys-EP antibody. A TUNEL assay was used to determine DNA fragmentation.Results and ConclusionsExcept for the one or two cell layers that constitute the lasting endosperm in the mature seed, ricinosomes were found in suspensor and endosperm cells. These cells were also the site of morphological abnormalities, including misshapen and fragmented nuclei, vesiculation of the cytosol, vacuole collapse and cell wall disorganization. It is proposed that, in suspensor and endosperm cells, the early detection of Cys-EP in ricinosomes predicts the occurrence of PCD during late seed development.