Characterization and Antineoplasic Effect of Extracts Obtained from Pleurotus sajor-caju Fruiting Bodies

• Published in: Applied biochemistry and biotechnology Vol. 160; no. 8; pp. 2265 - 2274
• Main Authors: Dalonso, Nicole, Souza, Rafaela, Silveira, Márcia Luciane Lange, Ruzza, Ângelo Adolfo, Wagner, Theodoro Marcel, Wisbeck, Elisabeth, Furlan, Sandra Aparecida
• Format: Journal Article
• Language: English
• Published: New York New York : Humana Press Inc 01.04.2010; Humana Press Inc; Springer; Springer Nature B.V
• Subjects: Adult; animal disease models; Animals; anticarcinogenic activity; Antineoplastic Agents - chemistry; Antineoplastic Agents - therapeutic use; Biochemistry; Biological and medical sciences; Biotechnology; Body Weight; Carcinoma, Ehrlich Tumor - drug therapy; Carcinoma, Ehrlich Tumor - pathology; Cell Extracts - chemistry; Cell Extracts - therapeutic use; Cells; Chemistry; Chemistry and Materials Science; Child; Extraction processes; Female; fruiting bodies; Fruiting Bodies, Fungal - chemistry; Fundamental and applied biological sciences. Psychology; galactose; glucose; Humans; mannose; Mice; Neoplasm Transplantation; nuclear magnetic resonance spectroscopy; Nuclear Magnetic Resonance, Biomolecular; Pharmacology; plant extracts; Pleurotus - chemistry; Pleurotus - cytology; Pleurotus sajor-caju; polysaccharides; Polysaccharides - chemistry; Polysaccharides - therapeutic use; Spectroscopy, Fourier Transform Infrared; Bioactive molecules; Characterization; Antineoplasic activity; Polysaccharidic fractions; -; Fungi; Pleurotus sajor-caju; Pleurotus; Extract; Basidiomycota
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-009-8678-9

Fungi of the Pleurotus genus present a great industrial interest due to their possibility of producing pharmacological compounds, pigments, aromas, organic acids, polysaccharides, enzymes, vitamins, amino acids, etc. Among the therapeutic products, we can highlight those with antineoplasic activity, attributed to the fungi cell wall components. Based on this, the objective of this work was to study the antineoplasic capacity of the polysaccharidic fractions obtained from Pleurotus sajor-caju fruiting bodies. Female Swiss mice were inoculated with the Ehrlich ascitic tumor (5 × 10⁶ cells/animal) in ascitic form. The polysaccharidic fractions were administered intraperitoneally, during a 6-day period. Fractions FI and FII presented a lower volume of ascitic liquid (3.1 and 1.8 mL, respectively) and a higher reduction in the number of neoplasic cells present in the ascitic liquid (86.2% and 85%, respectively), when compared to the positive control (group inoculated with the tumor but without treatment). These fractions were characterized in terms of monosaccharide composition. Glucose was the major component detected, followed by galactose and mannose. The anomeric carbon configuration of the β-glucan was confirmed by the ¹³C NMR (δ 103.7). Substituted and free C3 and C6 were also detected. Protein bands were confirmed through infrared analysis.