Application of a New Gene-Cell Construct Based on the Olfactory Mucosa Escheating Cells Transduced with an Adenoviral Vector Encoding Mature BDNF in the Therapy of Spinal Cord Cysts

• Published in: Bulletin of experimental biology and medicine Vol. 172; no. 5; pp. 617 - 621
• Main Authors: Karsuntseva, E. K., Fursa, G. A., Sosnovtseva, A. O., Voronova, A. D., Chadin, A. V., Semkina, A. S., Stepanova, O. V., Chekhonin, V. P.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.03.2022; Springer; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Biomedicine; Brain-derived neurotrophic factor; Cell Biology; Cell proliferation; Comparative analysis; Cysts; Genes; Health aspects; Internal Medicine; Laboratory Medicine; Motor activity; Mucosa; Neurophysiology; Olfactory epithelium; Pathology; Spinal cord; Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine); Transplantation; adenovector; olfactory mucosa ensheathing cells; gene therapy; spinal cord cysts; cell therapy
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-022-05446-x

A gene-cell construct based on rat olfactory mucosa ensheathing cells transduced with an adenoviral vector encoding a mature form of brain neurotrophic factor (mBDNF) was transplanted into post-traumatic cysts of rat spinal cord. Transplantation of the gene-cell construct improved motor activity of the hind limbs and reduced the size of cysts in some animals. However, comparison of the effects of transduced and non-transduced ensheathing cells revealed no significant differences. In parallel in vitro experiments, a decrease in the proliferation of transduced cells compared to non-transduced cells was observed. It is likely that mBDNF reduces proliferation of transduced cells, which can affect their efficiency. The therapeutic efficacy of the new gene-cell construct is most likely provided by the cellular component.