Mucilaginibacter kameinonensis sp., nov., isolated from garden soil

• Published in: International journal of systematic and evolutionary microbiology Vol. 58; no. 9; pp. 2046 - 2050
• Main Authors: Urai, Makoto, Aizawa, Tomoko, Nakagawa, Yasuyoshi, Nakajima, Mutsuyasu, Sunairi, Michio
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.09.2008; Society for General Microbiology
• Subjects: Bacterial Typing Techniques; Bacteriology; Bacteroidetes - classification; Bacteroidetes - genetics; Bacteroidetes - isolation & purification; Bacteroidetes - physiology; Base Composition; Biological and medical sciences; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Fatty Acids - analysis; Fundamental and applied biological sciences. Psychology; Genes, rRNA; Hydrogen-Ion Concentration; Japan; Locomotion; Microbiology; Miscellaneous; Molecular Sequence Data; Phospholipids - analysis; Phylogeny; Pigments, Biological - biosynthesis; Polysaccharides, Bacterial - biosynthesis; Quinones - analysis; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Sequence Homology, Nucleic Acid; Soil Microbiology; Systematics; Temperature; Japan; Garden; Soils; Bacteria; New species; Taxonomy
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.65777-0

1 Department of Applied Biological Sciences, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan 2 Biological Resource Center (NBRC), Department of Biotechnology, National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan 3 Environmental Program, Nihon University Advanced Research Institute for the Sciences and Humanities, 12-5 Goban-cho Chiyoda, Tokyo 102-8251, Japan Correspondence Michio Sunairi sunairi.michio{at}nihon-u.ac.jp An extracellular polysaccharide-producing bacterium, strain SCK T , was isolated from a soil sample taken from Kameino, Fujisawa, Japan. The isolate was Gram-negative and cells were non-motile, irregular-shaped rods that grew optimally at 25 °C and grew between pH 5 and 8. Strain SCK T contained MK-7 as the major isoprenoid quinone, iso-C 15 : 0 and C 16 : 1 7 c and/or iso-C 15 : 0 2-OH as the major fatty acids and sphingolipids, with d-17 : 0 as the main dihydrosphingosine. Flexirubin-type pigments were also present. The DNA G+C content was 43.7 mol%. On the basis of 16S rRNA gene sequence analysis, strain SCK T was shown to belong to the genus Mucilaginibacter . The 16S rRNA gene sequence similarity between strain SCK T and the two type strains of Mucilaginibacter was 93 %. The results of physiological and biochemical tests allowed phenotypic differentiation of the strain from published Mucilaginibacter species. Therefore, strain SCK T represents a novel species, for which the name Mucilaginibacter kameinonensis sp. nov. is proposed. The type strain is SCK T (=NBRC 102645 T =KCTC 22227 T ). Abbreviations: EPS, extracellular polysaccharide; PAH, polyaromatic hydrocarbon These authors contributed equally to this work. Present address: RIKEN (The Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain SCK T is AB330392 .