Tetragenococcus koreensis sp. nov., a novel rhamnolipid-producing bacterium

• Published in: International journal of systematic and evolutionary microbiology Vol. 55; no. 4; pp. 1409 - 1413
• Main Authors: Lee, Myungjin, Kim, Myung Kyum, Vancanneyt, Marc, Swings, Jean, Kim, Seong-Hye, Kang, Myung Suk, Lee, Sung-Taik
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.07.2005; Society for General Microbiology
• Subjects: Bacterial Typing Techniques; Bacteriology; Base Composition; Biological and medical sciences; DNA, Bacterial - analysis; DNA, Ribosomal - analysis; Fatty Acids - analysis; Fermentation; Fundamental and applied biological sciences. Psychology; Genes, rRNA; Glycolipids - biosynthesis; Gram-Positive Cocci - chemistry; Gram-Positive Cocci - classification; Gram-Positive Cocci - genetics; Gram-Positive Cocci - isolation & purification; Korea; Microbiology; Miscellaneous; Molecular Sequence Data; Nucleic Acid Hybridization; Phenotype; Phylogeny; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Systematics; Tetragenococcus halophilus; Tetragenococcus koreensis; Vegetables - microbiology; Korea; Taxonomy; Bacteria; Tetragenococcus
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.63448-0

1 NeoPharm Co., Ltd, BVC-307, KRIBB, 52 Oeun-dong, Yuseong-gu, Daejeon 305-333, Republic of Korea 2 Environmental and Molecular Microbiology Laboratory, Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea 3 BCCM/LMG Bacteria Collection, Laboratory of Microbiology, Faculty of Sciences, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium Correspondence Sung-Taik Lee e_stlee{at}kaist.ac.kr A Gram-positive, non-motile, non-spore-forming coccus (strain JS T ) was isolated from kimchi (a traditional Korean food) and investigated using a polyphasic taxonomic approach. The 16S rRNA gene sequence similarity between strain JS T and its closest relative, Tetragenococcus halophilus IAM 1676 T , was 98·1 %. The level of DNA–DNA relatedness between the two strains was 9·7 %. Strain JS T had a DNA G+C content of 38·3 % and a cellular fatty acid profile containing 16 : 0, 18 : 1 and cyclo fatty acids. Phylogenetic data and genomic and phenotypic features demonstrated that strain JS T represents a novel species, for which the name Tetragenococcus koreensis sp. nov. is proposed. The type strain is JS T (=KCTC 3924 T =DSM 16501 T =LMG 22864 T ). The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain JS T is AY690334 . A scanning electron micrograph of cells of strain JS T is available as supplementary material in IJSEM Online.