Comparison of Biochemical Parameters and Pathomorphological Changes in Rats Receiving Standard and High-Fat Diets during Modeling of Streptozotocin Diabetes

• Published in: Bulletin of experimental biology and medicine Vol. 172; no. 3; pp. 368 - 372
• Main Authors: Borozdina, N. A., Shaykhutdinova, E. R., Palikov, V. A., Palikova, Yu. A., Kazakova, E. N., Sadovnikova, E. S., Kazakov, V. A., Sintsova, O. V., Dyachenko, I. A.
• Format: Journal Article
• Language: English
• Published: New York Springer US 2022; Springer; Springer Nature B.V
• Subjects: Animals; Biochemical analysis; Biological products; Biomedical and Life Sciences; Biomedicine; Blood Glucose; Cell Biology; Cholesterol; Diabetes; Diabetes mellitus; Diabetes Mellitus, Experimental - drug therapy; Diabetes therapy; Diet; Diet, High-Fat - adverse effects; High fat diet; Humans; Hyperglycemia; Internal Medicine; Laboratory Medicine; Liver; Liver diseases; Microvasculature; Nephropathy; Pathology; Phosphates; Rats; Rats, Wistar; Rodents; Streptozocin; Triglycerides; Type 2 diabetes; hyperglycemia; streptozotocin (STZ); diabetes mellitus; rats; high-fat diet (HFD)
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-022-05394-6

The use of a high-fat diet, along with streptozotocin administration, can provide more profound insight into the mechanism of development of complications in diabetes, as well as their treatment. High-fat diet given over 3 weeks before intraperitoneal injection of streptozotocin in a dose of 40 mg/kg promoted the appearance of hyperglycemia in Wistar rats. The biochemical analysis of blood serum revealed increased levels of urea, triglycerides, cholesterol, AST, ALT, and concentration of inorganic phosphates and K + ions in the high-fat diet group in comparison with the control. Both the biochemical analysis of the blood and histological analysis showed more pronounced abnormalities in rats receiving high-fat diet in comparison with animals receiving standard ration. These changes are the early markers for the development of nephropathy, impaired liver function, and microvascular disorders typical of patients with diabetes mellitus.