Heterogeneous Expression of Proangiogenic and Coagulation Proteins in Gliomas of Different Histopathological Grade

• Published in: Pathology oncology research Vol. 27; p. 605017
• Main Authors: Wojtukiewicz, Marek Z, Mysliwiec, Marta, Matuszewska, Elwira, Sulkowski, Stanislaw, Zimnoch, Lech, Politynska, Barbara, Wojtukiewicz, Anna M, Tucker, Stephanie C, Honn, Kenneth V
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media SA 31.03.2021; Frontiers Media S.A
• Subjects: Angiogenesis; Angiogenic Proteins - metabolism; Antibodies; Antigens; Blood Coagulation Factors - metabolism; Blood vessels; Brain cancer; Brain Neoplasms - metabolism; Brain Neoplasms - pathology; Brain research; Glioma; Glioma - metabolism; Glioma - pathology; Humans; Hypoxia; Kinases; Medical prognosis; Neoplasm Grading; Neovascularization, Pathologic - metabolism; Permeability; Proteins; Society Journal Archive; Tumors; Vascular endothelial growth factor; United States > US; D-dimers; tissue factor; blood coagulation; VEGF; angiogenesis; fibrin; glial tumors 3; bFGF
• ISSN: 1532-2807; 1219-4956; 1532-2807
• DOI: 10.3389/pore.2021.605017

Brain gliomas are characterized by remarkably intense invasive growth and the ability to create new blood vessels. Angiogenesis is a key process in the progression of these tumors. Coagulation and fibrinolysis factors play a role in promoting angiogenesis. The aim of the study was to evaluate the expression of proangiogenic proteins (VEGF and bFGF) and hemostatic proteins (TF, fibrinogen, fibrin, D-dimers) associated with neoplastic cells and vascular endothelial cells in brain gliomas of various degrees of malignancy. Immunohistochemical tests were performed using the ABC method with the use of mono- and polyclonal antibodies. The obtained results indicated that both neoplastic cells and vascular endothelial cells in gliomas of various degrees of malignancy are characterized by heterogeneous expression of proteins of the hemostatic system and angiogenesis markers. The strongest expression of proangiogenic factors and procoagulant factors was demonstrated in gliomas of higher-grade malignancy.