Electrochemical DNA biosensors based on platinum nanoparticles combined carbon nanotubes

• Published in: Analytica chimica acta Vol. 545; no. 1; pp. 21 - 26
• Main Authors: Zhu, Ningning, Chang, Zhu, He, Pingang, Fang, Yuzhi
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 22.07.2005; Elsevier
• Subjects: Analytical chemistry; Biological and medical sciences; Biosensors; Biotechnology; Carbon nanotubes; Chemistry; Electrochemical DNA biosensor; Electrochemical methods; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; Methods. Procedures. Technologies; Platinum nanoparticles; Various methods and equipments; Carbon nanotubes; Electrochemical DNA biosensor; Platinum nanoparticles; Catalytic reaction; End group; Electrochemical method; Nanoparticle; Differential impulse voltamperometry; Carbon electrode; Electron transfer; Sensitivity; Chemical reaction; Platinum; Biosensor; DNA; Detection limit; Covalent bond; Oligonucleotide
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2005.04.015

Platinum nanoparticles were used in combination with multi-walled carbon nanotubes (MWCNTs) for fabricating sensitivity-enhanced electrochemical DNA biosensor. Multi-walled carbon nanotubes and platinum nanoparticles were dispersed in Nafion, which were used to fabricate the modification of the glassy carbon electrode (GCE) surface. Oligonucleotides with amino groups at the 5′ end were covalently linked onto carboxylic groups of MWCNTs on the electrode. The hybridization events were monitored by differential pulse voltammetry (DPV) measurement of the intercalated daunomycin. Due to the ability of carbon nanotubes to promote electron-transfer reactions, the high catalytic activities of platinum nanoparticles for chemical reactions, the sensitivity of presented electrochemical DNA biosensors was remarkably improved. The detection limit of the method for target DNA was 1.0 × 10 −11 mol l −1.