Layer by layer composite film of dimyristoyl-phosphatidylcoline and bacteriorhodopsin fabricated by multilayer molecular thin film method using fatty acid and lipid

• Published in: Thin solid films Vol. 518; no. 2; pp. 600 - 605
• Main Authors: Tsujiuchi, Yutaka, Furuya, Keigo, Matsumoto, Jun, Makino, Yukinobu, Ito, Manabu, Masumoto, Hiroshi, Goto, Takashi
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 30.11.2009; Elsevier
• Subjects: Bacteriorhodopsin; Blodgett film; Condensed matter: structure, mechanical and thermal properties; Cross-disciplinary physics: materials science; rheology; Electron, ion, and scanning probe microscopy; Exact sciences and technology; Fatty acid; Langmuir; Liquid phase epitaxy; deposition from liquid phases (melts, solutions, and surface layers on liquids); Materials science; Membrane protein; Methods of deposition of films and coatings; film growth and epitaxy; Multilayer molecular thin film; Phosphatidylcoline; Physics; Structure and morphology; thickness; Structure of solids and liquids; crystallography; Surfaces and interfaces; thin films and whiskers (structure and nonelectronic properties); Thin film structure and morphology; Bacteriorhodopsin; Phosphatidylcoline; Membrane protein; Fatty acid; Langmuir; Blodgett film; Multilayer molecular thin film; Composite film; Fourier transform spectroscopy; Amorphous thin film; Lipids; Infrared spectra; Fatty acids; Membrane proteins; Surface structure; Thin films; Multilayers; Langmuir-Blodgett films; Reflection spectrum; Amorphous hydrogenated material; Absorption spectra
• ISSN: 0040-6090; 1879-2731
• DOI: 10.1016/j.tsf.2009.07.049

A composite film of dimyristoyl-phosphatidylcoline (DMPC) and bacteriorhodopsin (BR) was fabricated by multilayer molecular thin film method using fatty acid and lipid on a quartz substrate or a hydrogenated amorphous silicon thin film. FTIR reflection absorption spectrums and UV absorption spectrums of the films were characterized on the detail of surface structure of the films. The spectroscopic data exhibited a specific layer by layer interaction of BR and environmental molecule DMPC above fatty acid. Especially, 4 layer composite LB films DMPC and BR exhibited an entirely different feature of IR reflection absorption spectrum depend on fatty acid species.