A Method of Direct Quantitation of Lactobacillus spp. in Intestinal Contents Based on Real-Time PCR

• Published in: Bulletin of experimental biology and medicine Vol. 171; no. 6; pp. 794 - 797
• Main Authors: Markova, Yu. M., Stetsenko, V. V., Polyanina, A. S.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.10.2021; Springer; Springer Nature B.V
• Subjects: Analysis; Bacteria; Bacterial Load; Biomedical and Life Sciences; Biomedicine; Calibration; Cell Biology; DNA Primers - chemical synthesis; DNA Primers - genetics; Feces - microbiology; Gastrointestinal Microbiome - genetics; Gastrointestinal system; Humans; Internal Medicine; Intestine; Laboratory Medicine; Lactobacillus; Lactobacillus acidophilus - classification; Lactobacillus acidophilus - genetics; Lactobacillus acidophilus - isolation & purification; Lactobacillus paracasei - classification; Lactobacillus paracasei - genetics; Lactobacillus paracasei - isolation & purification; Lactobacillus plantarum - classification; Lactobacillus plantarum - genetics; Lactobacillus plantarum - isolation & purification; Methods; Microbiota; Microbiota (Symbiotic organisms); Microorganisms; Pathology; Polymerase Chain Reaction - methods; Polymerase Chain Reaction - standards; Quantitation; RNA, Ribosomal, 16S - genetics; Sensitivity and Specificity; lactobacilli; gut microbiota; real-time PCR
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-021-05318-w

The method of direct quantitation of Lactobacillus spp. and L. acidophilus in intestinal contents based on real-time PCR was developed. It does not require culturing and allows estimating the number of living lactobacilli cells (measured in lg CFU) in absolute quantitative PCR format.