A novel NMDA receptor positive allosteric modulator that acts via the transmembrane domain

• Published in: Neuropharmacology Vol. 121; pp. 204 - 218
• Main Authors: Wang, Tzu-Ming, Brown, Brandon M., Deng, Lunbin, Sellers, Benjamin D., Lupardus, Patrick J., Wallweber, Heidi J.A., Gustafson, Amy, Wong, Evera, Volgraf, Matthew, Schwarz, Jacob B., Hackos, David H., Hanson, Jesse E.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 15.07.2017
• Subjects: Allosteric Regulation - drug effects; Allosteric Regulation - genetics; Binding Sites - drug effects; Binding Sites - genetics; Calcium - metabolism; Dose-Response Relationship, Drug; Doxycycline - pharmacology; Electric Stimulation; Excitatory Amino Acid Agents - chemistry; Excitatory Amino Acid Agents - pharmacology; Glutamic Acid - pharmacology; Glycine - metabolism; HEK293 Cells; Humans; Membrane Potentials - drug effects; Membrane Potentials - genetics; NMDA receptor; Patch-Clamp Techniques; Positive allosteric modulator; Protein Domains - drug effects; Protein Domains - genetics; Pyrimidinones - chemistry; Pyrimidinones - pharmacology; Receptors, N-Methyl-D-Aspartate - genetics; Receptors, N-Methyl-D-Aspartate - metabolism; Sulfonamides - chemistry; Sulfonamides - pharmacology; Synaptic Transmission - drug effects; Synaptic Transmission - genetics; Synaptic Transmission - physiology; Transfection; Transmembrane domain; Ifenprodil (PubChem CID; GNE-8016 (PubChem CID; iGluR; 118009479; 20938953; 4231127; Transmembrane domain; 22432385; TMD; GNE-3419 (PubChem CID; LBD; ATD; GNE-9278 (PubChem CID; Positive allosteric modulator; NAM; NMDA receptor; 3689; PAM; CIQ (PubChem CID
• ISSN: 0028-3908; 1873-7064
• DOI: 10.1016/j.neuropharm.2017.04.041

Ionotropic glutamate receptors (iGluRs) mediate fast excitatory neurotransmission and are key nervous system drug targets. While diverse pharmacological tools have yielded insight into iGluR extracellular domain function, less is known about molecular mechanisms underlying the ion conduction gating process within the transmembrane domain (TMD). We have discovered a novel NMDAR positive allosteric modulator (PAM), GNE-9278, with a unique binding site on the extracellular surface of the TMD. Mutation of a single residue near the Lurcher motif on GluN1 M3 can convert GNE-9278 modulation from positive to negative, and replacing three AMPAR pre-M1 residues with corresponding NMDAR residues can confer GNE-9278 sensitivity to AMPARs. Modulation by GNE-9278 is state-dependent and significantly alters extracellular domain pharmacology. The unique properties and structural determinants of GNE-9278 reveal new modulatory potential of the iGluR TMD. •GNE-9278 is a NMDAR positive allosteric modulator that acts at the GluN1 TMD.•GNE-9278 acts on activated NMDARs to increase peak current and agonist affinity.•TMD point mutations can convert GNE-9278 modulation from positive to negative.•TMD point mutations can confer GNE-9278 potentiation to AMPARs.•GNE-9278 modulation is state-dependent and alters extracellular domain pharmacology.