Palleronia marisminoris gen. nov., sp. nov., a moderately halophilic, exopolysaccharide-producing bacterium belonging to the 'Alphaproteobacteria', isolated from a saline soil

• Published in: International journal of systematic and evolutionary microbiology Vol. 55; no. 6; pp. 2525 - 2530
• Main Authors: Martinez-Checa, Fernando, Quesada, Emilia, Martinez-Canovas, M. Jose, Llamas, Inmaculada, Bejar, Victoria
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.11.2005; Society for General Microbiology
• Subjects: Bacteriochlorophyll A - analysis; Bacteriology; Base Composition; Biological and medical sciences; DNA, Bacterial - chemistry; DNA, Bacterial - isolation & purification; DNA, Ribosomal - chemistry; DNA, Ribosomal - isolation & purification; Fatty Acids - analysis; Fundamental and applied biological sciences. Psychology; Microbiology; Molecular Sequence Data; Palleronia marisminoris; Polysaccharides, Bacterial - biosynthesis; Potassium - metabolism; Proteobacteria; Quinones - analysis; Rhodobacteraceae - classification; Rhodobacteraceae - isolation & purification; Rhodobacteraceae - physiology; Rhodobacteraceae - ultrastructure; RNA, Ribosomal, 16S - analysis; RNA, Ribosomal, 16S - genetics; Roseivivax halodurans; Roseivivax halotolerans; Roseobacter; Saline Solution, Hypertonic - pharmacology; Sequence Analysis, DNA; Sodium - metabolism; Soil Microbiology; Systematics; Soils; New genus; Halophily; Taxonomy; Salinity
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.63906-0

Microbial Exopolysaccharide Research Group, Department of Microbiology, Faculty of Pharmacy, Campus Universitario de Cartuja, University of Granada, 18071 Granada, Spain Correspondence Victoria Béjar vbejar{at}ugr.es Strain B33 T is a moderately halophilic, exopolysaccharide-producing, Gram-negative, non-motile rod isolated from a hypersaline soil bordering a saline saltern on the Mediterranean seaboard in Murcia (Spain). The bacterium is chemoheterotrophic and strictly aerobic. It contains a pink pigment but does not synthesize bacteriochlorophyll a . It requires 0·66 M Na + , 0·1 M Mg 2+ and 0·1 M K + for optimum growth. It does not produce acid from carbohydrates. It cannot grow with carbohydrates, organic acids, sugars, alcohols or amino acids as sole sources of carbon and energy. Its major fatty-acids are 18 : 1 7 c (68·9 %) and 19 : 0 cyclo 8 c (12·8 %). The sole respiratory lipoquinone found in strain B33 T is ubiquinone-10. The DNA G+C content is 64·2 mol%. 16S rRNA gene sequence comparisons show that the isolate is a member of the Roseobacter clade within the class ‘ Alphaproteobacteria ’. The similarity values with Roseivivax halodurans and Roseivivax halotolerans are 88·2 and 88·0 % respectively and 92·2 % with Salipiger mucosus . DNA–DNA hybridization values with these species are <30 %. In the light of the polyphasic evidence gathered in this study it is proposed that the isolate be classified as a novel genus and species with the name Palleronia marisminoris gen. nov., sp. nov. The proposed type strain is strain B33 T (=CECT 7066 T =LMG 22959 T ). Abbreviations: EPS, exopolysaccharide; PHA, poly- -hydroxyalkanoate Published online ahead of print on 16 September 2005 as DOI 10.1099/ijs.0.63906-0. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Palleronia marisminoris B33 T is AY926462 . A more detailed phylogenetic tree based on 16S rRNA gene sequences is available as a supplementary figure in IJSEM Online.