Genetic analysis of kernel hardness in bread wheat using PCR-based markers

• Published in: Theoretical and applied genetics Vol. 103; no. 4; pp. 601 - 606
• Main Authors: GALANDE, A. A, TIWARI, R, AMMIRAJU, J. S. S, SANTRA, D. K, LAGU, M. D, RAO, V. S, GUPTA, V. S, MISRA, B. K, NAGARAJAN, S, RANJEKAR, P. K
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01.09.2001; Berlin Springer Nature B.V
• Subjects: Biological and medical sciences; Classical genetics, quantitative genetics, hybrids; Flour; Fundamental and applied biological sciences. Psychology; Genes; Genetics of eukaryotes. Biological and molecular evolution; Mutation; Proteins; Pteridophyta, spermatophyta; Spectrum analysis; Triticum; Vegetals; United States > US; India; Monocotyledones; Genetic marker; Quantitative character; Molecular marker; Line (plant); Genetic determinism; Random amplified polymorphic DNA marker; Cereal crop; Polymerase chain reaction; Gramineae; Angiospermae; Spermatophyta; Inbred; Locus; Triticum aestivum
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/pl00002915

In wheat, kernel hardness is a complex genetic trait involving various directly and indirectly contributing components such as kernel hardness per se, protein content, hectolitre weight and 1,000-kernel weight. In an attempt to identify DNA markers associated with this trait, 100 recombinant inbred lines (RILs) derived from a cross between a hard grain land-race, NP4, and a soft grain variety, HB 208, were screened with 100 ISSR and 360 RAPD primers. Eighteen markers were assigned to seven linkage groups covering 223.6 cM whereas 11 markers remained unlinked. A multiple-marker model explained the percentage of phenotypic variation for kernel hardness as 20.6%, whereas that for protein content, hectolitre weight and 1,000-kernel weight was 18.8%, 13.5% and 12.1%, respectively. Our results indicate that phenotypic expression of kernel hardness is controlled by many QTLs and is interdependent on various related traits.[PUBLICATION ABSTRACT]