Steroidobacter denitrificans gen. nov., sp. nov., a steroidal hormone-degrading gammaproteobacterium

• Published in: International journal of systematic and evolutionary microbiology Vol. 58; no. 9; pp. 2215 - 2223
• Main Authors: Fahrbach, Michael, Kuever, Jan, Remesch, Markko, Huber, Birgit E, Kampfer, Peter, Dott, Wolfgang, Hollender, Juliane
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.09.2008; Society for General Microbiology
• Subjects: Anaerobiosis; Bacterial Typing Techniques; Bacteriology; Biological and medical sciences; Catalase - metabolism; Chromatiaceae; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Ectothiorhodospiraceae; Fatty Acids - metabolism; Fundamental and applied biological sciences. Psychology; Gammaproteobacteria - classification; Gammaproteobacteria - genetics; Gammaproteobacteria - isolation & purification; Gammaproteobacteria - metabolism; Genes, rRNA; Glutamic Acid - metabolism; Gonadal Steroid Hormones - metabolism; Hydrogen-Ion Concentration; Locomotion; Microbiology; Miscellaneous; Molecular Sequence Data; Nitrates - metabolism; Oxidoreductases - metabolism; Phospholipids - analysis; Phylogeny; Quinones - analysis; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Sequence Homology, Nucleic Acid; Sewage - microbiology; Sodium Chloride - metabolism; Spores, Bacterial - cytology; Systematics; Temperature; Hormone; Bacteria; New genus; New species; Taxonomy
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.65342-0

1 Swiss Federal Institute of Aquatic Science and Technology, Eawag, Überlandstr. 133, PO Box 611, CH-8600 Dübendorf, Switzerland 2 Bremen Institute for Materials Testing, Foundation Institute for Materials Science, Paul-Feller-Str. 1, D-28199 Bremen, Germany 3 Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität Wien, Veterinärplatz 1, A-1210 Vienna, Austria 4 Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, Heinrich-Buff-Ring 26–32 (IFZ), D-35392 Giessen, Germany 5 Institute of Hygiene and Environmental Medicine, RWTH Aachen University, Pauwelsstr. 30, D-52074 Aachen, Germany Correspondence Juliane Hollender juliane.hollender{at}eawag.ch A denitrifying bacterium, designated strain FS T , was isolated from anoxic digested sludge on oestradiol [17 β -oestra-1,3,5(10)-triene-3,17-diol] or testosterone (17 β -hydroxyandrost-4-en-3-one) as the sole source of carbon and energy with nitrate as the electron acceptor. Strain FS T represents the first known bacterium to grow anaerobically on both oestradiol (C-18) and testosterone (C-19). Steroidal hormones were degraded completely by nitrate reduction to dinitrogen monoxide, which was further reduced to dinitrogen in stationary-phase cultures. Gram-negative cells were slightly curved rods, 0.3–0.5 x 0.6–1.6 µm in size, motile, non-fermentative, non-spore-forming and catalase- and oxidase-positive, showing optimal growth at pH 7.0, 28 °C and 0.1 % (w/v) NaCl. Beside steroidal hormones, the bacterium utilized only a narrow range of organic substrates with nitrate as the electron acceptor, including several fatty acids and glutamate. No aerobic or anaerobic growth occurred on liquid or solid complex media. Phylogenetic analysis of the 16S rRNA gene sequence showed that strain FS T has no known close relatives and represents a distinct lineage within the Gammaproteobacteria . Together with the genera Nevskia , Hydrocarboniphaga , Solimonas and Sinobacter (less than 88 % 16S rRNA gene sequence similarity to strain FS T ), it forms a phylogenetic cluster separated from the families Chromatiaceae , Ectothiorhodospiraceae and Xanthomonadaceae . The quinone system of strain FS T consisted exclusively of ubiquinone Q-8. The dominant polar lipids were diphosphatidylglycerol and phosphatidylethanolamine. Spermidine in combination with putrescine and traces of sym -homospermidine were the basic polyamines. The major fatty acids detected in testosterone- or heptanoate-grown cells were C 15 : 0 and C 17 : 1 8 c , minor hydroxylated fatty acids were C 11 : 0 3-OH and C 12 : 0 3-OH. The G+C content of the DNA was 61.9 mol%. Based on the high 16S rRNA gene sequence divergence and different phenotypic properties from previously described gammaproteobacteria in combination with chemotaxonomic data, strain FS T is considered to represent a new genus and species, for which the name Steroidobacter denitrificans gen. nov., sp. nov. is proposed. The type strain of Steroidobacter denitrificans is FS T (=DSM 18526 T =JCM 14622 T ). The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain FS T is EF605262 .