Expression and activity of glutathione S-transferases and catalase in the shrimp Litopenaeus vannamei inoculated with a toxic Microcystis aeruginosa strain

• Published in: Marine environmental research Vol. 75; pp. 54 - 61
• Main Authors: Gonçalves-Soares, Daniela, Zanette, Juliano, Yunes, João S., Yepiz-Plascencia, Gloria M., Bainy, Afonso C.D.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Oxford Elsevier Ltd 01.04.2012; Elsevier
• Subjects: Algal toxins; Animal and plant ecology; Animal, plant and microbial ecology; Animals; antioxidants; Bacterial Toxins - toxicity; Base Sequence; biochemical mechanisms; Biological and medical sciences; Biomarker; Catalase; Catalase - genetics; Catalase - metabolism; Crustacea; Environmental toxicology; enzyme activity; Fundamental and applied biological sciences. Psychology; gene expression; Gene Expression Regulation, Enzymologic - drug effects; glutathione; Glutathione S-transferase; glutathione transferase; Glutathione Transferase - genetics; Glutathione Transferase - metabolism; hepatopancreas; Hepatopancreas - drug effects; Hepatopancreas - enzymology; humans; Invertebrates; Litopenaeus vannamei; Marine Toxins - toxicity; Microcystin; Microcystins - toxicity; Microcystis; Microcystis aeruginosa; Molecular Sequence Data; Penaeidae - drug effects; Penaeidae - enzymology; Penaeidae - genetics; Pollution; Protein Isoforms - genetics; Protein Isoforms - metabolism; Sea water ecosystems; shrimp; Synecology; toxicity; Water Pollutants, Chemical - toxicity; wildlife; Environmental toxicology; Microcystis aeruginosa; Pollution; Biomarker; Catalase; Algal toxins; Microcystin; Litopenaeus vannamei; Glutathione S-transferase; Toxicity; Biological marker; Macrura; Crustacea; Marine environment; Toxicology; Glutathione transferase; Cyanobacteria; Bacteria; Decapoda; Shrimp; Enzyme; Transferases; Penaeidae; Toxin; Peroxidases; Arthropoda; Oxidoreductases; Invertebrata
• ISSN: 0141-1136; 1879-0291
• DOI: 10.1016/j.marenvres.2011.07.007

Microcystin (MC) produced during cyanobacteria blooms is notably toxic to human and wildlife. Conjugation with reduced glutathione (GSH) by glutathione S-transferase (GST) and the antioxidant enzymes defenses (e.g. catalase, CAT) are important biochemical defense mechanisms against MCs toxicity. We investigated the enzymatic activity of CAT and GST and the gene expression levels of CAT and eight GST isoforms in the hepatopancreas of the globally farmed shrimp Litopenaeus vannamei 48-h after injection with a sub-lethal dose of 100μgkg−1 of a toxic Microcystis aeruginosa extract. MCs caused up-regulation for GSTΩ, μ and a MAPEG isoform, by 12-, 2.8- and 1.8-fold, respectively, and increases in the total GST enzyme activity and CAT enzyme activity. The study points to the importance of further characterization for the L. vannamei GST isoforms and GST/CAT post-translational regulation processes to better understand the key mechanisms involved in the shrimp’s defense against MC exposure. ► CAT and GST were analyzed in Litopenaeus vannamei treated with Microcystin. ► MCs up-regulated Ω, μ and microsomal GST isoforms and down-regulated CAT. ► Total GST and CAT enzyme activities were increased in the MCs treated shrimp. ► GSH conjugation could be an important MCs detoxification mechanism in shrimp. ► High CAT activity suggests enhanced ROS production during MCs biotransformation.