Nocardia jiangxiensis sp. nov. and Nocardia miyunensis sp. nov., isolated from acidic soils

• Published in: International journal of systematic and evolutionary microbiology Vol. 55; no. 5; pp. 1921 - 1925
• Main Authors: Cui, Qingfeng, Wang, Liming, Huang, Ying, Liu, Zhiheng, Goodfellow, Michael
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.09.2005; Society for General Microbiology
• Subjects: Bacterial Typing Techniques; Bacteriology; Biological and medical sciences; China; DNA, Bacterial - analysis; DNA, Ribosomal - analysis; Fundamental and applied biological sciences. Psychology; Genes, rRNA; Hydrogen-Ion Concentration; Microbiology; Miscellaneous; Molecular Sequence Data; Nocardia - classification; Nocardia - genetics; Nocardia - isolation & purification; Nocardia jiangxiensis; Nocardia nova; Nocardia pseudobrasiliensis; Nucleic Acid Hybridization; Phenotype; Phylogeny; Pinus; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Soil Microbiology; Systematics; Trees; China; Actinomycetales; Soils; Nocardia; Taxonomy; Bacteria; Nocardiaceae; Actinomycetes
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.63644-0

1 State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China 2 School of Biology, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK Correspondence Ying Huang huangy{at}im.ac.cn The taxonomic positions of two soil actinomycetes, strains 117 T and 43401 T , provisionally assigned to the genus Nocardia were determined in a polyphasic study. The organisms were found to have phenotypic properties typical of members of the genus Nocardia , and formed a distinct branch in the Nocardia 16S rRNA gene tree. It was evident from the phylogenetic data that the isolates were most closely, albeit loosely, associated with the type strains of Nocardia nova and Nocardia pseudobrasiliensis . However, all four of these organisms were readily distinguishable from one another using DNA–DNA relatedness and phenotypic data. It is evident from the genotypic and phenotypic data that the two isolates should be recognized as novel species of the genus Nocardia . It is proposed, therefore, that strains 117 T (=CGMCC 4.1904 T =JCM 12860 T ) and 43401 T (=CGMCC 4.1905 T =JCM 12861 T ) be classified in the genus Nocardia as the type strains of Nocardia miyunensis sp. nov. and Nocardia jiangxiensis sp. nov., respectively. Published online ahead of print on 13 May 2005 as DOI 10.1099/ijs.0.63644-0. The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains 117 T and 43401 T are AY639901 and AY639902 , respectively. Scanning electron micrographs of strains 117 T and 43401 T are available as a supplementary figure in IJSEM Online.